异常黑胆质成熟剂对人增生性瘢痕成纤维细胞TGF-β1表达的影响  

Influence of abnormal savdamunziq on expression of TGF-β1 in human hypertrophic scar fibroblasts

作　　者：孔孟龙 李楠[1] 马涛[1] 马少林[1] 

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054

出　　处：《中华中医药杂志》2015年第6期2168-2170,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81260291)~~

摘　　要：目的:观察不同浓度异常黑胆质成熟剂(ASMq)对体外培养的人增生性瘢痕成纤维细胞(HSFBs)转化生长因子-βl(TGF-β1)蛋白及m RNA表达的影响,在分子水平上探讨其抑制增生性瘢痕生长的作用机制。方法:体外培养人HSFBs,选取第3-5代细胞采用含不同浓度异常黑胆质成熟剂(200、400、600μg/m L)的DMEM培养液培养48h,运用RT-PCR技术检测各组TGF-β1 m RNA表达水平。运用Western bolt方法测量TGF-β1蛋白表达水平。结果:与对照组相比,3个药物剂量组HSFBs TGF-β1蛋白及m RNA的表达均减少(P<0.05),药物浓度为400μg/m L时效果最佳,且TGF-β1蛋白及m RNA表达的变化具有一致性。结论:ASMq有抑制体外培养的人HSFBs TGF-β1表达的作用,初步探讨了ASMq发挥抑制增生性瘢痕生长的作用机制,为ASMq药物的开发利用提供理论依据。Objective： To investigate the influence of abnormal savdamunziq （ASMq） on the protein and RNA expression of transforming growth factor-betal （TGF-β1） in human hypertrophic scar fibroblasts （hHSFs） and clarify the mechanism of inhibiting the growth of hypertrophic scar at the molecular level. Methods： HHSFs were isolated from tissue specimens and cultured in vitro, and the ceils at 3rd to 5th generation were selected for the further researches, which were cultured in DMEM culture media containing with different concentrations of ASMq （200, 400, 600lag/mL）. After culturing for 48 hours, the protein and mRNA expression of TGF-β1 in cells was examined by using Western blot and RT-PCR respectively. Results： Compared with the blank control group, the protein and mRNA expressions of TGF-β1 in cells cultured in the three doses of drug groups were decreased （P〈0.05）, and the effect of drug concentration at 4001ag/mLwas the best, meanwhile, the changes in protein and mRNA expression of TGF-β1 were consistency. Conclusion： ASMq could inhibit the expression of TGF-β1 in hHSFs cultured in vitro. This paper preliminarily discussed the action mechanism of inhibiting hypertrophic scar with ASMq, and provided a theoretical basis for the development and utilization of ASMq.

关 键 词：异常黑胆质成熟剂 增生性癜痕成纤维细胞 转化生长因子-Β1 RT-PCR WESTERN BLOT 

分 类 号：R285[医药卫生—中药学]