黄芪多糖对肝纤维化大鼠TGF-β1/Smads信号通路的影响  

Effects of astragalan on liver fibrosis rat in TGF-β1/Smads signal pathway

作　　者：黄进[1] 张晨[2] 詹菲[2] 张进[2] 

机构地区：[1]广州中医药大学门诊部,广州510405 [2]广州中医药大学基础医学院,广州510006

出　　处：《中华中医药杂志》2015年第6期2184-2186,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：教育部高等学校博士学科点专项科研基金项目(No.20114425120015);广东省普通高校重点实验室开放基金资助项目(No.AAF433111A27)~~

摘　　要：目的:观察黄芪多糖(APS)对四氯化碳(CCl4)诱导肝纤维化大鼠转化生长因子-β1(TGF-β1)/Smads信号通路的影响。方法:采用CCl4诱导大鼠肝纤维化模型,取大鼠血清检测肝功能;Masson染色比较肝纤维化程度;免疫组化法测肝组织TGF-β1蛋白表达;Real-time PCR分析肝组织TGF-β1、Smad3、Smad4以及Smad7的表达。结果:与模型组比较,APS各剂量组的血清肝功水平、肝纤维化程度明显降低,肝组织TGF-β1、Smad3、Smad4表达量显著降低,Smad7表达量显著增高。结论:APS对CCl4诱导的大鼠肝纤维化有显著的干预作用,其作用机制可能与调节TGF-β1/Smads信号通路有关。Objective： To investigate effects of astragalan on liver fibrosis rat induced by carbon tetrachloride （CCl4） in TGF-β1/Smads signal pathway. Methods： The rat model of experimental liver fibrosis was established by CCI4, and liver function was tested by serum of rat. The comparition of the fibrosis degrees was observed using Masson staining. The protein expressions of TGF-β1/Smads was detected by immunohistochemistry method; The mRNA of TGF-β1, Smad3, Smad4 and Smad7 were assessed using Real-time PCR. Results： Compared with the model group, the level of liver function and the degree of fibrosis in serum of Alas group lowered obviously, the expression of TGF-β1, Smad3 and Smad4 was significantly decreased, and the expression of Smad7 was significantly increased. Conclusion： Astragalan has significantly interfering effect on liver fibrosis rat induced by carbon tetrachloride （CCl4）, and its mechanism may be related to regulation TGFq3/Smad signaling pathway.

关 键 词：肝纤维化 黄芪多糖 四氯化碳 信号通路 

分 类 号：R285.5[医药卫生—中药学]