AlloMax补片植入大鼠腹壁不同层面后组织相容性研究  

A biocompatibility study on Allomax mesh implanted in different planes of abdominal wall in a rat model

作　　者：王海亮[1] 孙宏杰[2] 王秀芹[2] 菅凤国[1] 

机构地区：[1]潍坊医学院研究生院,山东省,261042 [2]昌邑市人民医院普通外科

出　　处：《中华普通外科杂志》2015年第5期391-394,共4页Chinese Journal of General Surgery

摘　　要：目的 探讨AlloMax补片植入大鼠腹壁不同层面后的组织相容性.方法 SD大鼠随机分为腹壁肌筋膜前组（Onlay组）、肌后组（Sublay组）和腹膜内组（IPOM组）,分别在补片植入后1、3、6个月观察补片粘连和皱缩等情况,并对成纤维细胞的长入、支架降解、细胞外基质沉积和血管长入数目进行定量分析.结果 大体观察表明Onlay组和Sublay组补片与腹壁整合好于IPOM组,前2组补片在各时间点都显示平展、无皱缩和移位,与腹腔组织和脏器无粘连.6个月时补片大部分或全部与宿主腹壁组织整合.IPOM组则出现网膜与补片粘连,随时间延长而增加,6个月时补片大部分未与腹壁组织整合,并可见补片皱缩和移位.组织学观察：与Onlay组和IPOM组相比,Sublay组在补片植入3个月时,其内出现脂肪细胞.6个月时成纤维细胞和新生血管长入数量也明显少于前2组.另外在各个时间点支架降解和细胞外基质沉积水平也明显低于前二组.结论 Allomax补片植入大鼠腹壁肌筋膜前层的组织相容性优于肌筋膜后层和腹膜内层.Objective To evaluate biocompatibility of Allomax mesh implanted in different planes of abdominal wall in a rats model.Methods SD rats were randomly assigned to the profacial group （Onlay group）,the retro-rectus group （Sublay group） versus the intraperitoneal group（IPOM group） according to different abdominal wall planes the mesh implanted,Adhesion and shrinkage of the mesh were observed,and quantitative measurements were conducted in fibroblast ingrowth,scaffold degradation,extracellular matrix deposition and numbers of vascular ingrowth after 1,3 and 6 months mesh was implanted.Results Macroscopic observation showed both Onlay and Sublay groups was superior to IPOM group in abdominal wall integration,which included shrinkage,relocation and adhesion of the mesh at all the time points,and most or whole of the mesh had incorporated with host abdominal wall at 6 months.Most of the mesh had not incorporated with host abdominal wall and shrinkage and relocation of the mesh were found in IPOM group at 6 months.Microscopic investigation showed lipocytes appeared in the mesh in Sublay group at 3 months,and numbers of ingrowth of fibroblast and neovascularization in Sublay group were significantly less than in Onlay and IPOM group at 6 months.Scaffold degradation and extracellular matrix deposition were remarkably less in Sublay group in comparison with Onlay group and IPOM group after 1,3 and 6 months.Conclusions Biocompatibility of AlloMax mesh implanted in profacial plane of abdominal wall was superior to implanted in retro-rectus plane and intraperitoneal plane as showed in a rat model.

关 键 词：生物相容性材料 腹壁 组织相容性 

分 类 号：R318.08[医药卫生—生物医学工程]