基于成像技术采用内膜损伤法构建动脉易损斑块动物模型方法研究  

Animal model of arterial vulnerable plaque for imaging study using balloon-injury method

作　　者：段小艺[1] 方佳[2] 邓蕾[1] 尚进[1] 商静[1] 宋法亮[2] 

机构地区：[1]西安交通大学第一医院影像科,陕西西安710061 [2]石河子大学医学院第二附属医院影像科

出　　处：《实用放射学杂志》2015年第5期840-843,共4页Journal of Practical Radiology

基　　金：国家自然科学基金（地区科学基金）资助项目

摘　　要：目的：建立适用于分子影像学研究的家兔腹主动脉易损斑块模型。方法选择健康雄性新西兰大白兔随机分为高脂饮食＋球囊损伤组（A 组）,高脂饮食组（B 组）和普通饲料喂养组（C 组）,采用超声与磁共振成像动态观察腹主动脉粥样斑块形成情况,与血脂水平及病理学指标对照。结果 A 组在观察第4周即可见腹主动脉斑块形成,第8周斑块迅速增多,至12周斑块数量达到22个,均为软斑块；B 组于第8周发现少量斑块,12周斑块总数仅为7个；C 组全程未见斑块形成。血脂变化与成像所见斑块形成过程基本一致。镜下斑块中可见巨噬细胞与平滑肌细胞显著增多,提示为易损斑块。结论高脂饮食＋球囊损伤法是构建动脉易损斑块动物模型用于成像研究的有效方法。Objective To establish animal model of arterial vulnerable plaques for molecular imaging study.Methods Fifteen New Zealand white rabbits were randomly divided into high lipid diet＋balloon-injury group （A）,high lipid diet group （B）and regular diet group （C）.Ultrasound （US）and magnetic resonance （MR）imaging were used to dynamically observe the formation of plaque in abdominal aorta. Results were compared with blood lipid level and pathological indicators.Results At 4 weeks,several plaques could be seen in group A.The plaque number increased rapidly and reached to 22 at 12 weeks,which was in parallel with the change of blood lipid. Only a few plaques were observed in group B,while no vulnerable plaque was revealed in group C.All the plaques were judged to be soft plaques on US and MR images,which was consistent with the macrophages gathering and smooth muscle cell proliferating in plaques.Conclusion High lipid diet＋balloon-injury is an ideal method to build animal model for molecular imaging of atherosclerotic plaque.

关 键 词：动脉易损斑块 超声 磁共振成像 动物模型 

分 类 号：R332[医药卫生—人体生理学] R445.1[医药卫生—基础医学]