rhKGF、CBLB502和WR2721对放射性口腔黏膜炎的防治效果观察  

Comparative study of the effects of rh KGF, CBLB502 and WR2721 on radiation-induced oral mucositis

作　　者：杨超[1] 王丽梅[2] 崔宇[2] 徐铁柱[2] 赵致维 李中堂[2] 王俊可 善亚君[2] 柳晓兰[2] 唐红卫[3] 从玉文[2] 

机构地区：[1]新乡医学院研究生院,河南新乡453003 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]武警总医院消化内科,北京100390

出　　处：《解放军医学杂志》2015年第5期420-424,共5页Medical Journal of Chinese People's Liberation Army

摘　　要：目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体重变化,另外20只小鼠利用1%甲苯胺蓝染色法观察照射小鼠舌组织溃疡情况,HE染色观察舌组织病理学改变,Ki-67免疫组化检测黏膜角化上皮细胞增殖情况。结果与照射对照组相比,rh KGF和WR2721给药组小鼠30d存活率显著提高,体重恢复较快,角化上皮细胞增殖显著,未发生明显的口腔黏膜炎;CBLB502组小鼠的存活率、体重变化及组织病理学指标与照射对照组比较差异无统计学意义(P>0.05)。结论 rh KGF和WR2721对放射性口腔黏膜炎有较好的防治作用,而CBLB502并不能减轻放射性口腔黏膜炎的发生。Objective To compare the protective effect of rh KGF, CBLB502 and WR2721 on radiation-induced oral mucositis（ROM）. Methods Fifty male 6-8-week-old C57BL/6J mice were randomly divided into normal group, irradiation control group, rh KGF group, CBLB502 group, and WR2721 group（n=10 each）. The 30-day sur vival rate and change in body weight of mice that had received 17 Gy irradiation of head and neck area were recorded. In another group of 20 mice, 1% toluidine blue staining and HE staining were used to observe oral ulcers and pathological changes in the tongue tissue. The proliferation of keratinocy te cells was assessed by Ki-67 immunohistochemistr y. Results Compared with the irradiation control group, administration of rh KGF and WR2721 could significantly improve the 30-day survival rate, accelerate the recovery of body weight, and promote the proliferation of keratinized epithelial cells of mice after irradiation, without inducing obvious oral mucositis. However, There was no significant difference between CBLB502 group and irradiation control group in survival rate, body weight and pathological changes in tongue tissues of mice. Conclusion rh KGF and WR2721 could alleviate ROM and improve the survival of mice, while CBLB502 has no such effect.

关 键 词：辐射损伤 实验性 辐射防护剂 成纤维细胞生长因子7 鞭毛蛋白 氨磷汀 

分 类 号：R818.898[医药卫生—放射医学]