碳纤维增强纳米羟基磷灰石/聚酰胺66复合材料与动物体内骨组织的相容性  

In vivo animal study on osteal histocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites

作　　者：鹿鸣[1,2] 张雪松[1] 徐辉[3] 胡文浩[1] 杨晓青[1] 

机构地区：[1]解放军总医院骨科,北京市100853 [2]总参管理保障部北极寺老干部服务管理局门诊部,北京市100191 [3]山东聊城市人民医院骨科,山东省聊城市252004

出　　处：《中国组织工程研究》2015年第16期2523-2528,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:碳纤维增强纳米羟基磷灰石/聚酰胺66复合材料相对于羟基磷灰石材料和其他纳米羟基磷灰石二元复合材料在力学强度、韧性和弹性模量等方面有了明显的提高,可用于承受载荷部位的骨缺损修复。目的:评价新型生物复合材料碳纤维增强纳米羟基磷灰石/聚酰胺66在骨组织的生物相容性。方法:使用巴马小型猪8只,在每只巴马小型猪的胸椎建立骨缺损模型并植入碳纤维增强纳米羟基磷灰石/聚酰胺66复合材料,在植入后8,16,24周处死动物并取材,行骨密度检测,胸椎椎体缺损处行苏木精-伊红染色。在植入前、植入后1,8周取血,查肝肾功能并进行对比。结果与结论:植入后8周,椎体松质骨断端封闭,复合材料被肉芽组织包裹,椎体松质骨断端可见软骨细胞,界面间软骨成骨活跃。植入后16周,肉芽组织机化通过纤维内成骨方式生成新的骨组织并与骨端骨质大致融合。植入后24周,新生骨组织已成为成熟的板层骨,断端与复合材料结合紧密。植入复合材料的椎体骨密度在植入后8,16,24周的均数呈上升趋势(P<0.05),随着植入时间增长,成骨量增加。植入前后的肝肾功能差异无显著性意义。实验可以初步认为碳纤维增强纳米羟基磷灰石/聚酰胺66是一种组织相容性、生物活性好,无肝肾毒性的新型骨缺损修复材料。BACKGROUND： Compared with hydroxyapatite materials and other nano-hydroxyapatite composites, carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites have been significantly improved in the mechanical strength, toughness, elastic modulus and other aspects. It can be used for repairing bone defects of loading parts. OBJECTIVE： To investigate the biocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites in bone tissues. METHODS： Eight Bama mini pigs were taken to establish models of thoracic vertebral defects and implanted with carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites. At 8, 16 and 24 weeks after implantation,the animals were sacrificed, respectively, for bone mineral density detection and hematoxylin-eosin staining. Blood samples for kidney and liver function tests were taken before and 1 and 8 weeks after implantation. RESULTS AND CONCLUSION： Hematoxylin-eosin staining of bone samples showed that the materials could bond with the bone defect interface without rejection, and could induce osteogenesis of chondrocytes. At 8 weeks after surgery, the broken ends of cancellous bone closed and the composite material was wrapped by granulation tissues. At 16 weeks after surgery, granulation tissues were organized and new bone developed directly from fibroblast cells. The new bone tissues were nearly fused with the end of cancellous bone. At 24 weeks after surgery, new bone tissue became mature lamellar bone, and the end of cancellous bone was connected tightly with the composite material. Bone mineral density of the implanted vertebra showed an increase trend at 8, 16 and 24 weeks after implantation. Over time, the bone mass was increased. The liver and kidney function tests showed that there was no significant difference before and after implantation. It is preliminarily believed that the carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composite has excellent histocompatibility and bioactivity without hepatic toxicity and nephritic toxi

关 键 词：羟基磷灰石类 组织相容性 纳米粒 骨和骨组织 动物实验 生物材料 材料相容性 纳米羟基磷灰石 聚酰胺66 碳纤维 骨缺损 修复 

分 类 号：R318[医药卫生—生物医学工程]