灌注法制备全肾脏脱细胞基质支架的体内生物相容性  

In vivo biocompatibility of whole-kidney acellular matrix scaffolds prepared by perfusion method

作　　者：陈捷[1] 杨镜秋[1] 刘春晓[2] 

机构地区：[1]汕头大学医学院第二附属医院泌尿外科,广东省汕头市515041 [2]南方医科大学珠江医院泌尿外科,广东省广州市510282

出　　处：《中国组织工程研究》2015年第16期2529-2533,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省医学科学技术研究基金(A2012394)~~

摘　　要：背景:应用灌注法制备的大鼠全肾脏脱细胞基质支架具有良好的体外细胞相容性,但其体内生物相容性尚不明确。目的:应用灌注法制备大鼠全肾脏脱细胞基质支架,检测其体内生物相容性。方法:应用灌注法制备Wistar大鼠全肾脏脱细胞基质支架,进行以下实验:1急性毒性实验:在小鼠腹腔分别注射全肾脏脱细胞基质支架浸提液、生理盐水及苯酚。2溶血实验:将抗凝新西兰兔血分别与全肾脏脱细胞基质支架浸提液、生理盐水及蒸馏水混合。3热源实验:向新西兰兔耳缘静脉注射全肾脏脱细胞基质支架浸提液。4内皮刺激实验:在新西兰兔皮下注射全肾脏脱细胞基质支架浸提液,观察有无皮肤刺激反应。5皮下植入实验:将全肾脏脱细胞基质支架埋入新西兰兔背部皮下。结果与结论:全灌注法制备的Wistar大鼠全肾脏脱细胞基质支架无细胞残留,未引起全身毒性反应、急性溶血反应、热源反应及皮肤刺激反应,植入兔体内具有良好的组织相容性。说明大鼠全肾脏脱细胞基质材料在动物体内具有很好的生物相容性。BACKGROUND： The whole-kidney acellular matrix scaffold in rats prepared by perfusion method is proved to have good in vitro biocompatibility, but in vivo biocompatibility is still unclear. OBJECTIVE： To produce a whole-kidney acellular matrix scaffold in rats by perfusion method and to evaluate the in vivo biocompatibility of the scaffold. METHODS： The whole-kidney acellular matrix scaffold in Wistar rats was prepared by perfusion method and evaluated with the following tests. （1） Acute toxicity test： mice were subject to intraperitoneal injection of whole-kidney acellular matrix scaffold extract liquid, normal saline and phenol. （2） Hemolytic test： Anticoagulant blood samples from New Zealand rabbits were mixed with whole-kidney acellular matrix scaffold extract liquid, normal saline and distilled water, respectively. （3） Pyrogen test： Whole-kidney acellular matrix scaffold extract liquid was injected into the ear vein of New Zealand rabbits. （4） Intracutaneous stimulation test： Whole-kidney acellular matrix scaffold extract liquid was injected subcutaneously into New Zealand rabbits for observing whether there was a skin stimulus response. （5） Subcutaneous implantation test： The whole-kidney acellular matrix scaffold was implanted subcutaneously into the back of New Zealand rabbits. RESULTS AND CONCLUSION： There was no cell residual in the whole-kidney acellular matrix scaffold preparedby perfusion method, and no acute systemic toxicity, hemolytic reaction, pyrogen response, and skin stimulus respons, indicating the scaffold has a good histocompatibility in the rabbits. These findings suggest that the whole-kidney acellular matrix scaffolds in Wistar rats prepared by perfusion method have good in vivo biocompatibility.

关 键 词：组织工程 肾 急性毒性试验 生物材料 材料相容性 脱细胞基质 生物相容性 灌注法 肾脏 

分 类 号：R318[医药卫生—生物医学工程]