活动性结核患者单核细胞中B淋巴细胞诱导成熟蛋白1的mRNA水平降低  

Down-regulated expression of Blimp-1 mRNA in monocytes from patients with active tuberculosis

作　　者：刘艳华[1] 王若[1] 程小星[1] 

机构地区：[1]解放军第309医院全军结核病研究所全军结核病防治重点实验室结核病诊疗新技术北京市重点实验室,北京100091

出　　处：《细胞与分子免疫学杂志》2015年第7期949-952,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81071318)

摘　　要：目的研究活动性结核患者单核细胞中B淋巴细胞诱导成熟蛋白1(Blimp-1)的mRNA水平与结核分枝杆菌(MTB)潜伏感染者和健康者相比是否发生改变。方法收集活动性结核患者、结核菌潜伏感染者和和健康者的抗凝外周血,从外周血中分离并纯化单核细胞,荧光定量PCR检测单核细胞中Blimp-1 mRNA的水平。而后分别用MTB的H37Rv菌株全菌裂解液和ESAT-6/CFP-10的抗原多肽刺激健康者的单核细胞,荧光定量PCR检测刺激前后单核细胞中Blimp-1 mRNA的水平。结果与健康者相比,活动性结核患者、潜伏感染者单核细胞Blimp-1的mRNA水平显著降低;与未刺激的单核细胞相比,全菌裂解液刺激后的单核细胞Blimp-1的mRNA水平显著降低,而ESAT-6/CFP-10抗原多肽刺激后的单核细胞中Blimp-1的mRNA水平无显著变化。结论活动性结核患者和MTB潜伏感染者单核细胞Blimp-1的mRNA水平降低,MTB全菌裂解液刺激的人单核细胞中Blimp-1的mRNA水平降低。Objective To detect the expression Of B lymphocyte-induced maturation protein-1 （Blimp-l） mRNA in rnonocytes of patients with active tuberculosis and analyze the differences of the expression among the patients, latent tuberculosis infection subjects and healthy controls （HCs）. Methods Anticoagulated peripheral blood was collected from patients with active tuberculosis, latent tuberculosis infection subjects and HCs. Monocytes were isolated and purified from the peripheral blood. Fluorescence quantitative PCR was used to detect the mRNA level of Blimp-1 in monocytes. After the monocytes from HCs were stimulated with whole cell lysate of H37Rv MTB and pepticle pool of ESAT-6/CFP-10, respectively, the level of Blimp-1 mRNA was again detected by fluorescence quantitative PCR. Results The level of Blimp-1 mRNA in the monocytes from patients with active tuberculosis and latent tuberculosis infection subjects were significantly lower than that from HCs. Compared with the unstimulated monocytes, the level of Blimp-1 mRNA in the monocytes stimulated with whole cell lysate of H37Rv MTB significantly decreased, while the expression of Blimp-1 mRNA in the monocytes stimulated with ESAT-6/CFP-]0 was not significantly changed. Conclusion The level of Blimp-1 mRNA in the monocytes from patients with active tuberculosis and latent tuberculosis infection subjects was down-regulated, and the expression of Blimp-1 mRNA in the monocytes from HCs was also suppressed after stimulated with whole cell lysate of MTB.

关 键 词：结核分枝杆菌 活动性结核 B淋巴细胞诱导成熟蛋白1 单核细胞 

分 类 号：R521[医药卫生—内科学] R378.911[医药卫生—临床医学]