小鼠卵巢器官的胰岛素抵抗及中药隐丹参酮的调控作用  

Ovarian insulin resistance and regulation of traditional Chinese medicine cryptotanshinone in mice

作　　者：陈静[1] 李威[2] 吴奇[2] 匡洪影[3] 吴效科[2] 

机构地区：[1]黑龙江中医药大学附属第一医院普外二科,哈尔滨医学硕士150040 [2]黑龙江中医药大学附属第一医院妇科一科,哈尔滨150040 [3]黑龙江中医药大学附属第一医院妇科二科,哈尔滨150040

出　　处：《医学研究生学报》2015年第5期475-479,共5页Journal of Medical Postgraduates

基　　金：国家中医药行业科研专项(201107005)

摘　　要：目的卵巢器官胰岛素通路障碍与生殖机能异常密切相关,文中旨在探讨胰岛素抵抗对卵巢生殖功能的影响,并评估隐丹参酮对卵巢器官胰岛素抵抗的治疗作用。方法将30只小鼠随机分为等渗盐水组、地塞米松a组(等渗盐水+地塞米松)和隐丹参酮a组(等渗盐水+地塞米松+隐丹参酮),每组10只。对各组小鼠进行胰岛素抵抗程度检测,测量每分钟衰变数(disintegrations per minute,DPM)、排卵率及血清激素水平检测。并将20只雌性小鼠处死后所得卵巢随机分为4组,每组8只卵巢:空白对照组(空白培养液)、地塞米松b组(地塞米松)、隐丹参酮b组(地塞米松+隐丹参酮)、沃曼青霉素组(地塞米松+隐丹参酮+沃曼青霉素),进行小鼠卵巢器官的体外培养,检测卵巢葡萄糖摄取量及培养液激素水平。结果1小鼠卵巢发生胰岛素抵抗后,地塞米松a组与等渗盐水组肝、脂肪、卵巢组织氚标记葡萄糖含量比较,差异均有统计学意义(P<0.05);卵巢隐丹参酮a组较地塞米松a组明显上升[(2 805.2±921.5)DPM/mg vs(1 183.2±382.2)DPM/mg,P<0.01];2与地塞米松a组排卵数、平均黄体数[(37.5±7.0)个、(5.0±1.8)个)]比较,等渗盐水组[(26.3±5.9)个、(3.5±1.6)个]和隐丹参酮组[(27.2±6.3)个、(3.8±0.9)个]均降低(P<0.05)。3等渗盐水组和隐丹参酮a组雌二醇、孕酮水平较地塞米松a组均显著降低(P<0.05)。4地塞米松a组P-AKT2蛋白分子表达量较等渗盐水组显著降低[(0.39±0.01)vs(0.77±0.03),P<0.05]。5体外培养卵巢组织中葡萄糖摄取量,空白对照组、地塞米松b组、隐丹参酮b组、沃曼青霉素组分别为(3 447.2±1245.2)、(1 183.2±382.2)、(2 805.2±921.5)、(1 364.2±292.3)DPM/mg。沃曼青霉素组和地塞米松b组较隐丹参酮b组、空白对照组均显著降低,差异均有统计学意义(P<0.01)。6与空白对照组比较,地塞米松b组培养液睾酮显著上升,孕酮水平显著下降,差异均有统计学意义(P<0.01)。�Objective The insulin pathways within ovary are in close relationship with female reproductive ability .The arti-cle was to observe the effects of ovarian insulin resistance on ovarian function and the effects of cryptotanshinone （CRY） on ovarian in-sulin resistance. Methods All mice were randomly divided into saline group （isotonic saline）, DEX a group （isotonic saline plus DEX）, CRY a group（isotonic saline plus DEX plus cryptotanshinone ）, 10 in each group.The degree of insulin resistance was tested on each rat, along with the measurement of disintergrations per minute （DPM）, ovulation rate and serum hormone level .After 20 fe-male mice were put to death , their ovaries were randomly divided into 4 groups：blank control group （ blank culture solution ） , DEX b group （DEX）, CRY b group（DEX plus cryptotanshinone ）, and WT group （DEX plus cryptotanshinone plus wortmannin ）, 8 in each group. Murine ovaries in each group were cultured in vitro to test the number of ovarian glucose and the serum hormone level . Results After in- sulin resistance took place in murine ovaries , significant statistical difference was found between DEX a group and saline group as to 2-deoxy-D-[1,2-3H]-glucose, liver, adipose and ovarian tissues （P〈0.05）.Ovaries in CRY a group increased significantly compared with DEX a group（[2805.2 ±921.5]DMP/mg vs [1183.2 ±382.2]DMP/mg, P＆lt;0.01 ）.Compared with DEX a group（37.5 ± 7.0, 5.0 ±1.8）, the ovulation rate and the average corpus luteum in saline group （26.3 ±5.9, 3.5 ±1.6） and CRY group （27.2 ± 6.3,3.8 ±0.9） decreased （P〈0.05）.In comparison to DEX a group, the estrogen and progestogen levels in saline group and CRY a group decreased significantly（P〈0.05）.The expression level of AKT2 protein in DEX a group was much lower than that in saline group（[0.39 ±0.01] vs [0.77 ±0.03], P〈0.05）.The glucose uptake in ovarian tissues cultured in vitro in blank control group , DEX b group, CRY b group and WT group was r

关 键 词：卵巢 胰岛素抵抗 雄激素 隐丹参酮 

分 类 号：R711.7[医药卫生—妇产科学]