类风湿关节炎模型大鼠滑膜组织中证实有髓样细胞诱发受体2的表达  

Triggering receptor expressed on myeloid cells 2 in synovial tissue of rheumatoid arthritis rats

作　　者：叶培[1] 李建华[2] 许锦煌 黄生辉[1] 刘贵旺 张玮琼 郑沛中 黄建荣[1,3] 

机构地区：[1]中山大学孙逸仙纪念医院骨科,广东省广州市510120 [2]广州医科大学生理学教研室,广东省广州市510182 [3]增城市人民医院骨科,广东省广州市511300

出　　处：《中国组织工程研究》2015年第18期2807-2813,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技基金(2010B031600184);广州市科技和信息化局重点项目基金(11C31120789);广东省自然科学基金(S2012010010629;S2013010013768)~~

摘　　要：背景:髓样细胞诱发受体2在类风湿关节炎活动期患者滑膜组织呈高表达,但其在类风湿关节炎发病机制中发挥的作用,目前并不清楚。目的:观察髓样细胞诱发受体2在牛源性Ⅱ型胶原诱导的关节炎大鼠模型滑膜组织中的表达。方法:制备胶原诱导关节炎模型大鼠,动态观察大鼠各项关节炎的活动指标和滑膜组织的病理学改变,RT-PCR法检测滑膜组织中髓样细胞诱发受体2、肿瘤坏死因子α、白细胞介素1β、白细胞介素10 mR NA的表达,Western blot及免疫组织化学法分别检测关节滑膜组织中髓样细胞诱发受体2的蛋白表达及定位。结果与结论:模型组大鼠免疫13 d后出现足爪红肿,关节炎指数评分逐渐升高(P<0.01),炎症高峰期在19-25 d,苏木精-伊红染色可见诱导性关节炎大鼠滑膜组织增生及炎性细胞浸润、软骨骨质破坏。与对照组相比,模型组大鼠关节滑膜髓样细胞诱发受体2的mR NA和蛋白的表达、肿瘤坏死因子α、白细胞介素1βmR NA表达均明显升高(P<0.05或P<0.01),白细胞介素10 mR NA表达显著降低(P<0.05)。结果证实,髓样细胞诱发受体2可能是类风湿关节炎模型大鼠发病过程中一个重要的炎症调节递质,其反应性升高在类风湿关节炎模型大鼠发病中发挥着重要作用。BACKGROUND：Triggering receptor expressed on myeloid cel s 2 （TREM-2） is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis stil remains unclear. OBJECTIVE：To explore the TREM-2 expression in the synovial tissue of col agen type II-induced arthritis rats. METHODS：The col agen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamical y observed. The mRNA levels of TREM-2, tumor necrosis factor-α,＆nbsp;interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively. RESULTS AND CONCLUSION：At day 13 after immunization, the paws of model rats appeared red and swel ing, the arthritis index scores were increased （P〈0.01）. At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of col agen-induced arthritis rats were proliferated and were infiltrated by inflammatory cel s, cartilage was destroyed. Compared with the control group, the expression of TREM-2 mRNA and protein, the mRNA levels of tumor necrosis factor-αand interleukin-1βin synovial tissue of the model rats were significantly increased （P〈0.05 or P〈0.01）, while interleukin-10 mRNA expression was significantly decreased （P〈0.05）. Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of col agen-induced arthritis.

关 键 词：实验动物 骨及关节损伤模型 髓样细胞诱发受体2 类风湿关节炎 胶原性关节炎 滑膜组织 炎症 肿瘤坏死因子α 白细胞介素1β 白细胞介素10 大鼠 广东省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]