前交叉韧带离断骨关节炎大鼠印第安刺猬蛋白以及Runt相关转录因子2的表达  

Expression of Indian hedgehog protein and Runt related transcription factor 2 in rats with osteoarthritis caused by anterior cruciate ligament transection

作　　者：孙一松 姚运峰[1] 方东[1] 荆珏华[1] 

机构地区：[1]安徽医科大学第二附属医院骨科,安徽省合肥市230000

出　　处：《中国组织工程研究》2015年第18期2820-2824,共5页Chinese Journal of Tissue Engineering Research

基　　金：安徽医科大学第二附属医院博士科研启动基金(2012BKJ014)~~

摘　　要：背景:印第安刺猬蛋白(Ihh)及其信号蛋白Gli1以及Runt相关转录因子2(Runx2)是骨关节炎密切相关的因子,在骨关节炎发病过程中,是引起关节退行性改变的重要原因之一。目的:探索Ihh,Gli1以及Runx2在骨关节炎发病过程中的作用。方法:将30只SD大鼠随机分成对照组(n=10)和模型组(n=20)。模型组大鼠取一侧膝关节行前交叉韧带离断建立骨关节炎模型,分别于造模后4周及12周,各取10只大鼠进行观察。对照组大鼠仅暴露前交叉韧带,于术后12周进行观察。结果与结论:与对照组相比,造模4周后可见骨关节炎模型大鼠膝关节出现软骨退变,膝关节软骨中Ihh,Gli1及Runx2表达水平明显增高,而造模12周后软骨退变进一步加重,但膝关节软骨中Ihh,Gli1及Runx2表达水平下降。提示Ihh,Gli1及Runx2在骨关节炎退变过程中起重要作用,其表达水平在骨关节炎的早期即明显升高,可作为骨关节炎防治研究中的评价指标。BACKGROUND：Indian hedgehog homolog （Ihh） protein and its signal protein Gli1, as wel as Runt related transcription factor 2 （Runx2） are closely related to the pathogenesis of osteoarthritis. The increased expression of these factors is one of the major causes of degenerative joint changes. OBJECTIVE：To explore the roles of Ihh, Gli1 and Runx2 in the development of osteoarthritis. METHODS：Thirty Sprague-Dawley rats were randomly divided into two groups：control group （n=10） and model group （n=20）. In the model group, rats received unilateral anterior cruciate ligament transection, to establish osteoarthritis model. Ten experimental rats were kil ed at 4 and 12 weeks after surgery respectively. Another 10 rats received unilateral knee arthrotomy as pseudo-operation controls and 10 pseudo-operation rats were kil ed at 12 weeks after surgery. RESULTS AND CONCLUSION：In the model group, cartilage degeneration was obvious at 4 weeks and became severer at 12 weeks after anterior cruciate ligament transection operation. Expression levels of Ihh, Gli1 and Runx2 in the cartilage were increased significantly at 4 weeks after operation, but decreased at 12 weeks after operation. Experimental findings indicate that Ihh, Gli1 and Runx2 play important roles in the development of osteoarthritis, and their expression levels are significantly increased at early stage of osteoarthritis, which can be regarded as the indicators in the prophylaxis and treatment research of osteoarthritis.

关 键 词：实验动物 骨及关节损伤模型 印第安刺猬蛋白 骨性关节炎 关节软骨退变 骨关节炎 动物模型 Runt相关转录因子2 

分 类 号：R318[医药卫生—生物医学工程]