经皮穿刺纤维环建立大鼠椎间盘源性疼痛模型  

A discogenic pain rat model induced by percutaneous puncture annulus

作　　者：吴志强[1] 周利君[2] 陈江波[1] 庄文权[1] 

机构地区：[1]中山大学附属第一医院介入放射科,广东省广州市510080 [2]中山大学疼痛研究中心,广东省广州市510080

出　　处：《中国组织工程研究》2015年第18期2831-2837,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81272041);广东省科技计划项目(2012B031800028)~~

摘　　要：背景:用于研究椎间盘源性疼痛的模型众多,采用经皮穿刺纤维环建立SD大鼠盘源性疼痛模型鲜见报道,利用微创方法建立的盘源性疼痛模型能减少开放性手术建模中组织创伤大的干扰因素。目的:建立一种操作简单、稳定性高且利于大样本量制作的SD大鼠盘源性疼痛模型,从行为学、影像学和分子生物学方面研究其时程变化。方法:SPF级SD雄性大鼠88只采用随机数表法分为对照组(n=10)、假手术组(n=34)和模型组(n=44)。模型组大鼠于X射线透视引导下经皮穿刺靶椎间盘纤维环;假手术组大鼠经皮穿刺椎旁组织,不穿刺纤维环。结果与结论:与对照组及假手术组相比,模型组大鼠双后足50%机械撤足阈值下降。模型组大鼠L5/6椎间盘退变较相邻椎间盘明显,其退变程度随时间延长有加重趋势。模型组大鼠背根神经节的降钙素基因相关肽从建模后第3天开始升高并在造模后第21天达到峰值,维持较高水平至第35天。模型组大鼠背根神经节的肿瘤坏死因子α也从建模后第3天开始升高并在造模后第14天达到峰值,维持较高水平至造模后第35天。表明X射线透视引导下穿刺SD大鼠纤维环建立的盘源性疼痛模型具有创伤小、稳定性好、干扰因素少等优点,可用于盘源性致痛的相关实验研究。BACKGROUND：There are many animal models used for studying discogenic pain, but percutaneous puncture annulus is rarely reported. Minimal y invasive approach to establish a discogenic pain model in Spraque-Dawley rats could reduce the interference factors of surgical trauma. OBJECTIVE：To establish a Spraque-Dawley rat model of discogenic pain by percutaneous puncture annulus, with easy operations, high stability and obtaining large-scale productions, and to confirm the model by the results of behavior, MRI and molecular biology. METHODS：Eighty-eight male Sprague-Dawley rats, of specific pathogen free level, were randomly divided into three groups, model group （n=44）, control group （n=10） and sham group （n=34）. In the model group, the annulus was percutaneously punctured under X-ray guidance;while rats in the sham group were punctured at the paravertebral tissue, rather than the annulus. RESULTS AND CONCLUSION：The 50%mechanical withdrawal threshold of both hind paws in model group were reduced compared with control group and sham group. In the model group, the L 5/6 intervertebral disc degeneration was apparently visible, and the degree of degeneration was aggravated along the time. In the＆nbsp;model group, the expression of calcitonin gene-related peptide in dorsal root ganglion of rats began to increase at 3 days post-operation and reached the peak at 21 days post-operation, then remained at high levels until the 35th day post-operation. The expression of tumor necrosis factor-αin dorsal root ganglion of rats increased at 3 days post-operation and reached the peak at 14 days post-operation, then remained at a higher level until the 35th day post-operation. The experiment result verifies that the discogenic pain model of Spraque-Dawley rats induced by percutaneous puncture annulus has the advantages of good stability and less trauma. The model can be used to study discogenic pain.

关 键 词：实验动物 骨及关节损伤模型 疼痛 X射线 经皮穿刺 椎间盘 肿瘤坏死因子α 降钙素基因相关肽 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]