灯盏花素干预糖尿病模型大鼠睾丸组织增殖细胞核抗原和c-fos的表达  

Breviscapine effects on the expression of proliferating cell nuclear antigen and c-fos in the testis of diabetic rat models

作　　者：龙玲莉[1] 郑淑慧[1] 李宇彬[2] 

机构地区：[1]中山大学附属第一医院转化中心实验室,广东省广州市510080 [2]中山大学附属第一医院,生殖医学中心广东省广州市510080

出　　处：《中国组织工程研究》2015年第18期2917-2922,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81100470)~~

摘　　要：背景:有研究表明灯盏花素可影响2型糖尿病大鼠的生殖能力,但其作用机制少有报道。目的:探讨灯盏花素对2型糖尿病大鼠睾丸增殖细胞核抗原和原癌基因c-fos表达的影响作用。方法:选取36只健康雄性大鼠随机分为对照组、模型组和灯盏花素组各12只。模型组和灯盏花素组采用链脲佐菌素连续腹腔注射建立2型糖尿病大鼠模型,大鼠血糖监测达到16.7 mmol/L作为建模标准,对照组大鼠予以同等容积的柠檬酸缓冲液单次腹腔注射。灯盏花素组采用灯盏花素注射液10 mg/(kg·d)连续4周腹腔注射,其他2组在相同时间注入等量生理盐水。结果与结论:干预4周后,血清睾酮检测、免疫组织化学染色和PCR检测结果显示,血清睾酮水平、增殖细胞核抗原、c-Fos蛋白及mR NA表达:对照组>灯盏花素组>模型组(P<0.05);血糖水平:对照组<灯盏花素组<模型组(P<0.05)。结果证实,灯盏花素可以通过增强增殖细胞核抗原和c-fos的表达,保护2型糖尿病大鼠的生殖功能。BACKGROUND：Breviscapine has been shown to impact the reproductive capacity in rats with type 2 diabetes mel itus, but few reports concerned its mechanism of action. OBJECTIVE：To study the effects of breviscapine on proliferating cel nuclear antigen and proto-oncogene c-fos expression in testis of type 2 diabetes mel itus rats. METHODS：Total y 36 healthy male rats were randomly divided into control group, model group and breviscapine group with 12 rats in each group. In the model group and breviscapine group, rat models of type 2 diabetes mel itus were established by continuous intraperitoneal injection of streptozotocin. Blood glucose reaching 16.7 mmol/L in rats was considered as the standard of model induction. In the control group, rats were given an equal volume of citrate buffer solution by single intraperitoneal injection. In the breviscapine group, rats were administered breviscapine 10 mg/（kg-d） for 4 consecutive weeks by intraperitoneal injection. Rats in the other two groups were injected with an equal volume of physiological saline at the same time point. RESULTS AND CONCLUSION：After 4 weeks of intervention, serum testosterone testing, immunohistochemistry and PCR results showed that serum testosterone levels, proliferating cel nuclear antigen, c-Fos protein and mRNA expression：control group〉breviscapine group〉model group （P〈0.05）;blood glucose concentration：the control group〈breviscapine group〈model group （P〈0.05）. Results confirmed that breviscapine can protect the reproductive function in type 2 diabetes mel itus rats by enhancing the expression of proliferating cel nuclear＆nbsp;antigen and c-fos.

关 键 词：组织工程 糖尿病 动物模型 基因病毒载体及相关因子模型 灯盏花素 2型糖尿病 睾丸增殖细胞核抗原 睾丸增殖细胞核抗原转录基因 C-FOS蛋白 原癌基因 大鼠 睾酮 链脲佐菌素 生殖功能 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]