早期糖尿病视网膜病变模型大鼠玻璃体腔注射安维汀后房水细胞因子变化  

Changes of cytokines in the aqueous humor following Avastin intravitreous injection in early diabetic retinopathy rats

作　　者：肖津安[1,2] 白清玉[3] 谢安明[1] 

机构地区：[1]西安交通大学医学院第一附属医院眼科,陕西省西安市710061 [2]西安市第四医院眼科,陕西省西安市710004 [3]西安市第四医院信息科,陕西省西安市710004

出　　处：《中国组织工程研究》2015年第18期2933-2939,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:关于抑制血管生成药安维汀治疗早期糖尿病视网膜病变大鼠的机制研究多数局限于血管内皮生长因子,而结缔组织生长因子和色素上皮衍生因子也在其中起重要的作用。目的:探讨安维汀玻璃体腔注射在早期糖尿病视网膜病变模型大鼠应用后房水细胞因子的变化及意义。方法:经链脲佐菌素诱导10周建立早期糖尿病视网膜病变模型大鼠,分别采用安维汀(1.25,2.5 mg)和生理盐水进行玻璃体腔注射。结果与结论:ELISA检测显示,与生理盐水注射组比较,两个安维汀注射组房水中血管内皮生长因子质量浓度降低,色素上皮衍生因子和结缔组织生长因子质量浓度增高(P<0.05),但两组间上述细胞因子的浓度差异无显著性意义(P>0.05)。结果证实,安维汀玻璃体腔注射在早期糖尿病视网膜病变大鼠应用促进新生血管的细胞因子血管内皮生长因子水平降低,抑制新生血管的细胞因子色素上皮衍生因子质量浓度升高,促进结缔组织生长因子水平升高。BACKGROUND：Studies on the inhibitory mechanism of Avastin for early diabetic retinopathy rats are mostly confined to the vascular endothelial growth factor, while connective tissue growth factor and pigment epithelium-derived factor also play an important role in the disease. OBJECTIVE：To evaluate the changed cytokines of aqueous humor and significance of Avastin intravitreous injection in early diabetic retinopathy rats. METHODS：Rat models of early diabetic retinopathy were established by inducing with streptozotocin for 10 weeks. Avastin （1.25 mg and 2.5 mg） and physiological saline were injected in the vitreous space. RESULTS AND CONCLUSION：Enzyme linked immunosorbent assay results revealed that compared with the physiological saline injection group, mass concentration of vascular endothelial growth factor was reduced, mass＆nbsp;concentrations of pigment epithelium-derived factor and connective tissue growth factor was increased in aqueous humor of the two Avastin injection groups （P0.05）. Results verified that Avastin intravitreous injection decreased vascular endothelial growth factor levels, decreased pigment epithelium-derived factor mass concentration and increased connective tissue growth factor levels in rat models of early diabetic retinopathy.

关 键 词：实验动物 基因病毒载体及相关因子模型 眼 糖尿病视网膜病变大鼠模型 安维汀 房水 细胞因子 血管内皮生长因子 色素上皮衍生因子 结缔组织生长因子 链脲佐菌素 

分 类 号：R318[医药卫生—生物医学工程]