不同停搏时间下HTK液对幼兔未成熟心肌的保护作用  

Myocardial Protection of HTK Solution in Immature Rabbit Myocardium at Different Cardiac Arrest Time

作　　者：张洪宇[1] 庄建[1] 

机构地区：[1]广东省心血管病研究所广东省人民医院广东省医学科学院小儿心外科,广州510080

出　　处：《中国胸心血管外科临床杂志》2015年第3期234-240,共7页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金："十二五"国家科技支撑计划项目(2011BAI11B22);广东省自然科学基金资助项目(S2011040005916)~~

摘　　要：目的比较不同停搏时间下,HTK心脏停搏液（HTK液）与St.ThomasⅡ心脏停搏液（STH液）对幼兔未成熟心肌的保护作用。方法根据不同停搏液及停搏时间将32只2~3周龄的新西兰大耳兔随机分为4组。SO组（8只）采用STH液停搏1 h,ST组（8只）采用STH液停搏2 h;HO组（8只）采用HTK液停搏1 h,HT组（8只）采用HTK液停搏2 h。比较不同停搏时间下,HTK液与STH液对幼兔未成熟心肌的保护效果。结果 SO组、HO组各8只,ST组、HT组各7只符合要求。HTK液与STH液在血流动力学及心肌酶（肌酸激酶同工酶、乳酸脱氢酶）方面差异无统计学意义（P〉0.05）,但HTK液能降低丙二醛（MDA）含量,维持超氧化物歧化酶（SOD）和Ca2＋-ATPase高活性,降低一氧化氮合酶（NOS）活性及NO含量（P〈0.05）,保护效果优于STH液。结论 HTK液对未成熟心肌保护优于STH液,但短时间（1 h）停搏下,STH液仍有良好表现。Objective To compare the myocardial protective effect of HTK solution and St.Thomas Ⅱ（STH） solution in immature rabbit myocardium at different cardiac arrest time. Methods According to cardioplegia and cardiac arrest time, 32 immature New Zealand white rabbits（aged 2-3 weeks） were randomly divided into four groups. A group SO（8 rabbits） underwent 1 hour cardiac arrest with STH solution, a group ST（8 rabbits） underwent 2 hours cardiac arrest with STH solution, a group HO（8 rabbits） underwent 1 hour cardiac arrest with HTK solution, a group Ht（8 rabbits） underwent 2 hours cardiac arrest with HTK solution. Compare the myocardial protective effect of HTK and STH solution in immature myocardium at different cardiac arrest time. Results The Langendorff models were successfully established in 30 cases（8 cases in the group SO and HO, 7 cases in the group ST and HT）. There were no statistical differences in hemodynamics and myocardial enzyme（CK-MB, LDH）（P〉0.05）, but HTK solution reduced the activity of nitric oxide synthase（NOS） and content of malonaldehyde（MDA） and NO, maintained high activity of superoxide dismutase（SOD） and Ca2＋-ATPase（P〈0.05）, performed more effective myocardial protection for immature myocardium. Conclusion HTK solution has more effective myocardial protection for immature myocardium than STH solution does, but STH solution still has good outcomes within short cardiac arrest time（1h）.

关 键 词：未成熟心肌 心肌保护 HTK液 心肌缺血再灌注损伤 

分 类 号：R725.4[医药卫生—儿科]