骨髓微环境对造血干细胞命运的调控  

Bone marrow microenvironment controls the “fate” of hematopoietic stem cells

作　　者：杜华[1] 师迎旭[1] 

机构地区：[1]内蒙古医科大学附属医院检验科,内蒙古自治区呼和浩特市010050

出　　处：《中国组织工程研究》2015年第14期2283-2290,共8页Chinese Journal of Tissue Engineering Research

基　　金：内蒙古医科大学科技百万工程基金(YKD2012KJBW006);内蒙古自治区高等学校科学研究项目(NJZY12145)~~

摘　　要：背景:研究表明造血微环境主要通过细胞-细胞、细胞-可溶性因子、细胞-细胞外基质3种模式对造血干细胞进行调控。目的:针对细胞-细胞、细胞-可溶性因子、细胞-细胞外基质3种模式调控过程中的几类信号通路和分子进行综述。方法:由第一作者分别以"Niche,HSC,VCAM-1,VLA-4,TPO,MPL,ECM,Integrin,N-cadherin,ANG-1,Tie2,ECM,VLA-5,Jagged-1,Notch,CXCL12,CXCR4,SCF,Kit,BMPs/TGF-β,TGF-βR,IFNα,IFNαR"为英文关键词,检索PubM ed英文数据库。通过阅读摘要,初步了解文献的大致内容,排除与本文无关或重复性研究以及观点陈旧的文献,共保留80篇文献进行综述。结果与结论:骨内膜表面的细胞、血管内皮细胞、血管周围细胞及骨髓腔内某些或某类未知的细胞或小分子,参与构成特化的造血干细胞微环境。造血干细胞驻留在造血微环境内并受其调控维持相对稳态。这一稳定状态的维持需要造血干细胞与造血微环境相互接触,并通过存在于细胞-细胞间黏附、细胞间可溶性细胞因子、细胞与细胞外基质之间的分子通路以及其他的一些重要分子例如Pten、骨桥蛋白来进行调节。BACKGROUND：Hematopoietic microenvironment regulates hematopoietic stem cel s mainly through the cel-cel , cel-soluble regulator and cel-extracel uar matrix modes. OBJECTIVE：To review the molecular mechanism underlying regulation of hematopoietic stem cel s under the cel-cel , cel-soluble factors and cel-extracel ular matrix modes. METHODS：A computer-based online search of PubMed database was performed to search related articles with the key words of“Niche, HSC, VCAM-1, VLA-4, TPO, MPL, ECM, Integrin, N-cadherin, ANG-1, Tie2, VLA-5, Jagged-1, Notch, CXCL12, CXCR4, SCF, Kit, BMPs/TGF-β, TGF-βR, IFNα, IFNαR”in English. Irrelevant or repetitive studies as wel as old literature were excluded, and final y 80 articles were included in result analysis. RESULTS AND CONCLUSION：Cel s on the endosteal surface, vascular endothelial cel s, perivascular cel s, and some unknown or certain cel s or smal molecules in the bone marrow cavity constitute the specialized microenvironment for hematopoietic stem cel s. Hematopoietic stem cel s in the hematopoietic microenvironment remain a relatively steady state, which is the result of mutual contact of hematopoietic stem cel s and hematopoietic microenvironment under the regulation of some important molecules, such as Pten and osteopontin, via the pathway between cel-intercel ular adhesions, intercel ular soluble factors, and cel s and extracel ular matrix.

关 键 词：干细胞 培养 干细胞龛(Niche) 造血干细胞 可溶性细胞因子 细胞外基质 

分 类 号：R318[医药卫生—生物医学工程]