石见穿活性部位的体外抗肿瘤作用研究  

In vitro anticancer activities of different extraction parts from Salvia chinensis beth

作　　者：徐敬宣 钱晓萍[1] 林黎[1] 薛姣[1] 胡静[1] 杨阳[1] 胡文静[1] 刘宝瑞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肿瘤中心,江苏南京210008

出　　处：《现代肿瘤医学》2015年第12期1632-1637,共6页Journal of Modern Oncology

基　　金：南京市卫生局重点项目(编号:ZKX09017)

摘　　要：目的:考察石见穿不同提取部位的体外抗肿瘤活性,从而确定其体外抗肿瘤作用的活性部位。方法:使用95%工业酒精对石见穿饮片进行提取,然后分别用石油醚、乙酸乙酯、氯仿和正丁醇依次对乙醇提取部位进行分段萃取,采用MTT法(四甲基偶氮唑蓝比色试验)测定各个提取部位对体外培养的5株人肿瘤细胞的增殖抑制作用。结果:在体外,氯仿部位和乙酸乙酯部位对于5株人肿瘤细胞株具有较强的增殖抑制作用。结论:氯仿部位、乙酸乙酯部位是石见穿发挥体外抗肿瘤作用的主要活性部位。Objective：To investigate in vitro anticancer activities of different extraction parts from Salvia chinensis beth. Methods：The extraction from Salvia chinensis beth with 95 % ethanol and then from the ethanol extraction part in turn with petroleum ether, ethyl acetate, trichloromethane and n - butanol was conducted. The inhibitory effects of these extraction parts on 5 human tumor lines were measured by MTY assay. Results：The trichloromethane extraction part and ethyl acetate extraction part from Salvia chinensis beth have more effective inhibitory effects on 5 human tumor lines in vitro. Conclusion： The trichloromethane extraction part and ethyl acetate extraction part from Salvia chinensis beth are main anticancer active parts in vitro.

关 键 词：石见穿 活性部位 MTT法 体外抗肿瘤作用 

分 类 号：R73-36[医药卫生—肿瘤]