刹毒草合剂对免疫抑制小鼠免疫功能的影响  

The effects of Shadu Cao Mixture on immune functions of immunosuppression mice

作　　者：卢舜飞[1] 汪亮亮[1] 卢俊婉[1] 刘丙进[1] 童应鹏[2] 程晓东[3] 叶挺梅[1] 

机构地区：[1]丽水学院医学院,浙江丽水323000 [2]浙江工业大学药学院,杭州310014 [3]同济大学生命科学与技术学院,上海200092

出　　处：《中国应用生理学杂志》2015年第3期266-269,共4页Chinese Journal of Applied Physiology

基　　金：浙江省自然科学基金项目(LQ12H29001);浙江省自然科学基金项目(LQ14H290001);浙江省科技厅重大科技专项社会发展项目(2011C03002)

摘　　要：目的:研究刹毒草合剂对免疫抑制小鼠免疫功能的影响。方法:BALB/C小鼠50只随机分为5组(n=10):空白组、模型组、刹毒草合剂低、中、高剂量组。除空白对照组外,其余各组小鼠腹腔注射环磷酰胺40 mg/kg建立免疫抑制模型。空白组和模型组灌胃生理盐水,刹毒草合剂低、中、高剂量组每天分别用刹毒草合剂10、20、40ml/kg灌胃,连续给药15 d后,检测小鼠外周血白细胞数和白介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)含量;检测小鼠脾脏指数、NK细胞活性。结果:与模型组比较,刹毒草合剂能显著增加外周血白细胞数和IL-2、TNF-α、IFN-γ含量、提高脾脏指数和NK细胞活性,差异均有统计学意义(P<0.05,P<0.01)。结论:刹毒草合剂可明显增强环磷酰胺诱导的免疫抑制小鼠的免疫功能。Objective： To investigate the effects of Shadu Cao Mixture（SDCM, traditional Chinese medicine） on immune functions of im- mtmosuppression mice. Methods： Fifty BALB/C mice were randomly divided into blank control group, model group, SDCM low-dose, mid- die-dose and high-dose group. Except the blank control group, other groups were intraperitoneal injected with cyclophosphamide （40 mg/kg） to establish mice model. The blank control group and model group received gavage administration with normal saline, while tile other groups received gavage administration with different doses of SDCM （ 10, 20, 40 ml/kg for 15 days） respectively. The number of leukocytes and serum levels of interleukin-2（IL-2）, tumor necrosis factor-a（TNF-a） and interferon-y（IFN-y） in peripheral blood, spleen in- dex, and the function of NK cells were measured. Results： Compared with the model group , SDCM increased tile number of lenkocytes and serum concentrations of IL-2, TNF-a and IFN-T in peripheral blood and improved the spleen index and the function of NK cells significantly （ P 〈0.05-0.01）. Conclusion： phamide. SDCM could remarkably enhance the immune functions of immunosuppression mice induced by cyclophos-

关 键 词：刹毒草合剂 免疫抑制 免疫功能 

分 类 号：R285.5[医药卫生—中药学]