养阴清肺方含药血清对^(60)Co照射后肺成纤维细胞转化生长因子β1及α-肌动蛋白表达的影响  

Effects of Medicated Serum with Yangyin Qingfei Fang( 养阴清肺 方) on the Expression of Transforming Growth Factor β1 and α-actin in Lung Fibroblast Cells with ^(60)Co Irradiated

作　　者：赵彪[1] 侯炜[2] 王兵[2] 杜欣颖[2] 王苏[2] 

机构地区：[1]四川省肿瘤医院 [2]中国中医科学院广安门医院

出　　处：《中医杂志》2015年第12期1061-1064,共4页Journal of Traditional Chinese Medicine

基　　金：北京市科技计划课题(D13100002213006)

摘　　要：目的探讨养阴清肺方治疗放射性肺纤维化的可能作用机制。方法将20只大鼠随机分为养阴清肺组[予养阴清肺方浓缩液(浓度0.97 g/ml)1 ml/100 g]、激素组[予醋酸泼尼松龙片水溶液(浓度0.125 g/ml)1 ml/100 g]、养阴清肺加激素组(养阴清肺方浓缩液、醋酸泼尼松龙片水溶液各0.5 ml/100 g)、模型组和未照射对照组(均给予生理盐水1 ml/100 g),每天早晚各灌胃1次,连续3天,第4天早上给药1 h后经腹主动脉取血制备含药血清。取人胚肺成纤维细胞接种于6孔板内,分为未照射对照组、模型组、养阴清肺组、激素组、养阴清肺加激素组,除未照射对照组外其余各组用钴60射线照射,剂量为8Gy。照射后将各组上清液吸弃,并分别换上相应的含药血清培养液,于24、48、72 h检测转化生长因子β1(TGF-β1)含量。细胞培养同前,于照射后24 h检测肺成纤维细胞α-肌动蛋白(α-SMA)的表达。结果模型组、养阴清肺组、激素组、养阴清肺加激素组在各个时间点比较TGF-β1表达水平均较未照射对照组显著升高(P<0.05)。在24 h时激素组、养阴清肺加激素组TGF-β1表达水平较模型组降低,在48 h时养阴清肺组、养阴清肺加激素组TGF-β1表达水平较模型组降低,72 h时养阴清肺组、激素组TGF-β1表达水平较模型组降低(P<0.05)。与模型组比较,激素组、养阴清肺组、养阴清肺加激素组α-SMA表达均明显降低(P<0.05);并且养阴清肺加激素组α-SMA表达明显低于养阴清肺组和激素组(P<0.05)。结论养阴清肺方能抑制照射后肺成纤维细胞分泌TGF-β1和α-SMA表达,可能是其治疗放射性肺纤维化的作用机制之一。Objective To explore the underlying possible mechanism of Yangyin Qingfei Fang（ YQF） in treating radioactive pulmonary fibrosis. Methods 20 Wistar rats were randomly divided into a YQF group（ given YQF concentrate with the concentration of 0. 97 g / ml,1 ml /100 g）,a hormone group（ given prednisolone acetate tablets aqueous solution with the concentration of 0. 125 g / ml,1 ml /100 g）,a YQF combined with hormone group（ given YQF concentrate combined with prednisolone acetate tablets aqueous solution,0. 5 ml /100 g respectively）,a model group and a non-irradiated group（ both given normal saline,1 ml /100 g）. All the animals were given with gavage twice a day（ morning and night） for three days. The medicated serum was prepared and produced by drawing blood from abdominal aorta one hour after medication on the fourth day morning. Human embryonic lung fibroblasts were inoculated in 6-well plates,and divided into a non-irradiated group,a model group,an YQF group,a hormone group,and a YQF combined with hormone group. Except the non-irradiated group,all groups were given Cobalt 60 irradiated with the dose of 8Gy. After irradiation,The supernate of all groups was sucked and discarded,and then the corresponding medicated serum culture mediums were added in,respectively. TGF-β1 content was determined at the 24 h,48 h and72 h. Cells were cultured ditto. 24 hours after irradiation,expression of α-SMA was tested. Results Expression of TGF-β1 was significantly increased at all times points in the model group,YQF group,hormone group and YQF combined with hormone group compared with that in the non-irradiated group（ P 〈 0. 05）. Compared with the model group,expression of TGF-β1 was obviously decreased in the hormone group and YQF combined with hormone group at the 24 h,in the YQF group and YQF combined with hormone group at 48 h,and in the YQF group and hormone group at 72 h（ P 〈 0. 05）. The expression of α-SMA was all significantly decreased in the hormone group,YQF group,and YQF combine

关 键 词：放射性肺纤维化 肺成纤维细胞 养阴清肺方 醋酸泼尼松龙片 转化生长因子Β1 Α-肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]