HPLC法检测2-氧-α-D-吡喃葡萄糖基抗坏血酸  

Determination of 2-O-α-d-glucopyranosyl l-ascorbic acid by HPLC

作　　者：张守雨 那可 赵文杰 胡海峰 

机构地区：[1]中国医药工业研究总院 [2]国药集团健康产业研究院有限公司,上海200040

出　　处：《分析试验室》2015年第6期732-734,共3页Chinese Journal of Analysis Laboratory

摘　　要：建立了高效液相色谱法检测维生素C衍生物2-氧-α-D-吡喃葡萄糖基抗坏血酸的方法。采用Waters AtlantisT3-C18色谱柱;流动相为25 mmol/L的KH2PO4-H3PO4缓冲溶液（p H 2.0）;流速为0.70 m L/min;柱温30℃;检测波长为238 nm。2-氧-α-D-吡喃葡萄糖基抗坏血酸质量浓度在0.0625~3 mg/m L范围内线性关系良好,相关系数〉0.99,回收率为100.2%,RSD为1.0%。A method was established for determination of 2-O-α-d-glucopyranosyl 1-ascorbic acid by highperformance liquid chromatography （HPLC）. The analysis was performed on a Atlantis T3-C18 column（4. 6 × 150 mm, 5 μm ） using 0. 025 mol/L potassium dihydrogen phosphate and phosphoric acid buffer solution （ pH 2.0） as the mobile phase at a flow rate of 0. 70 mL/min and 30 ℃ and detected at 238 nm by PDA. The calibration curve of 2-O-α-d-glucopyranosyl 1-ascorbic acid was linear in the range of 0. 0625 - 3 mg/mL, with the correlation coefficient （r） larger than 0.99. The recovery was 100. 2% with the relative standard deviation （RSD） of 1.0%.

关 键 词：2-氧-α-D-吡喃葡萄糖基抗坏血酸 高效液相色谱 检测 

分 类 号：O657.7[理学—分析化学]