羟基喜树碱柔性脂质体冻干剂的抗肿瘤作用研究  

Study on the antitumor effects of Lyophilized hydrocamptothecin flexible liposomes

作　　者：易跃能 吴文质 胡凯莉[3] 奉建芳[2,3] 

机构地区：[1]株洲市中心医院,湖南株洲430200 [2]湖南易能生物医药有限公司,湖南长沙410013 [3]上海中医药大学穆拉德中药现代化研究中心,上海201203

出　　处：《华西药学杂志》2015年第3期300-302,共3页West China Journal of Pharmaceutical Sciences

基　　金：科技部创新基金项目(12C26214304973);长沙市重点科技计划项目(K1113015-31)

摘　　要：目的研究羟基喜树碱(HCPT)柔性脂质体冻干剂(LHCPTFL)的抗肿瘤作用。方法以市售HCPT注射液为对照,采用MTT法分别考察LHCPTFL对肝癌Hep G-2、胃癌BGC-823及结肠癌LOVO细胞的体外抗肿瘤作用;并建立荷Hep G-2瘤裸鼠模型来考察LHCPTFL的体内抗肿瘤作用。结果与市售HCPT注射液相比,LHCPTFL对肝癌Hep G-2和胃癌BGC-823细胞的抗肿瘤作用较强,但对结肠癌LOVO细胞的抗肿瘤作用较弱,对裸鼠肝癌Hpe G-2移植瘤的抑制作用较好。结论将HCPT制备成柔性脂质体冻干剂可提高其抗肿瘤作用。OBJECTIVE To study the antitumor effects of Lyophilized hydrocamptothecin（ HCPT） flexible liposomes（ LHCPTFL） in vitro and in vivo. METHODS The marketing HCPT injections were used as control,and the in vitro antitumor effect of LHCPTFL against Hep G-2,BGC-823 and LOVO cells were evaluated by MTT method. A Hep G-2 cell bearing nude mice model was established to investigate the in vivo antitumor effect of LHCPTFL. RESULTS The antitumor effect of LHCPTFL to Hep G-2 and BGC-823 cells was significantly higher than the marketing HCPT injections,while the effect to LOVO cells was lower than the marketing HCPT injections. The antitumor effect of LHCPTFL to Hep G-2 cell bearing nude mice model was stronger than the marketing HCPT injections. CONCLUSION Lyophilized flexible liposomes enhanced the antitumor effect of HCPT.

关 键 词：羟基喜树碱 脂质体 冻干剂 抗肿瘤 Hep G-2细胞 BGC-823细胞 LOVO细胞 抑制 

分 类 号：R96[医药卫生—药理学]