HPLC分析不同种质的黄连  

Analysis of Coptidis rhizoma from the different germplasm resources by HPLC

作　　者：刘芳[1] 张浩[2] 青琳森[3] 武勇 

机构地区：[1]长治医学院,山西长治046000 [2]四川大学华西药学院,四川成都610041 [3]中国科学院成都生物研究所,四川成都610041 [4]山东宏济堂制药集团有限公司,山东济南250103

出　　处：《华西药学杂志》2015年第3期341-344,共4页West China Journal of Pharmaceutical Sciences

基　　金：科技部国家科技支撑计划重点项目(2007BA140B05);中国博士后科学基金第6批特别资助项目(2013T60863)

摘　　要：目的比较不同种质黄连的HPLC特征,有效区别黄连、西藏黄连、云南黄连、日本黄连等种质。方法对8种不同种质黄连样品进行HPLC指纹图谱分析及相似度评价,并测定其中7种小檗碱型生物碱的含量。结果不同种质黄连的HPLC图谱差异明显,表明黄连具有丰富的种质多样性。结论 HPLC比较法较全面地反映了不同种质黄连的差异,可用于药材的质量控制,可为黄连属植物的分类提供依据。OBJECTIVE To study the chemical characteristics of different germplasm Coptidis rhizoma by using HPLC comparison method,and to identify the different forms of Coptidis chinensis,C. teeta,C. teetoides,and C. Japonicon and so on. METHODS Eight different germplasm resources of C. rhizoma were analyzed by HPLC fingerprint and similarity evaluation was done,and the quaternary of seven amine-type alkaloids was determined. RESULTS The HPLC spectrograms of different germplasm C. rhizoma were significantly different,which indicated that the C. rhizoma had rich germplasm diversity. CONCLUSION HPLC comparison method could comprehensively reflect the differences of germplasm C. rhizoma,and can be used for quality control of the ingredients. The results has certain guiding significance in the taxonomy of C. rhizoma.

关 键 词：黄连 不同种质 高效液相色谱法 比较 指纹图谱 相似度 生物碱 质量控制 

分 类 号：R917[医药卫生—药物分析学]