正交试验法优化血人参中总黄酮的提取工艺  

Optimization of the extraction technology for total flavonoids from Indigofera stachyoides

作　　者：骆瀚超 郝小燕[1] 周威[1] 何茂秋[1] 马丽[1] 梁妍[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550025

出　　处：《华西药学杂志》2015年第3期358-360,共3页West China Journal of Pharmaceutical Sciences

基　　金：贵州省科技厅中药现代化基金项目(黔科合ZY字2013-3006);贵州省中医药管理局基金项目(QZYY2013-90)

摘　　要：目的优化血人参药材中总黄酮的提取工艺。方法对乙醇浓度、固液比、提取时间及提取次数进行单因素考察,再用正交试验进一步优化,以总黄酮纯度和提取率作为考察指标,筛选最佳提取工艺。结果血人参中总黄酮的最佳提取工艺为:用50%乙醇、固液比l∶30进行提取(1.5 h×2),血人参中总黄酮的平均提取率为7.42 mg·g-1。结论所选工艺稳定、可行,所得血人参中总黄酮的纯度和提取率均较高。OBJECTIVE To investigate the extraction technology for total flavonoids from Indigofera stachyoides. METHODS The ethanol aqueous concentration,solid-liquid ratio,extraction time and number of extraction were investigated respectively. And these were furtherly optimized by Orthogonal experimental test with purity and extraction rate of total flavonoids. RESULTS The optimum conditions for extraction process of the total flavonoids were： 50% of ethanol aqueous concentration,l∶30 of solid-liquid ratio,1. 5 h of extraction（ extracted twice）. The average extraction rate of total flavonoids from I. stachyoides was 7. 42 mg·g^-1under these conditions. CONCLUSION The extraction process for total flavonoids from I. stachyoides is stable and feasible. The purity and extractiom rate of total flavonoids from I. stachyoides were both higher than ever before.

关 键 词：血人参 总黄酮 提取工艺 正交试验 单因素考察 纯度 提取率 

分 类 号：R917[医药卫生—药物分析学]