北京鸭RIG-I基因的克隆及其生物信息学分析  

Cloning and bioinformatics analysis of Peking Duck Retinoic Acid Inducible Gene I

作　　者：王同淑 李传峰[2] 陈宗艳[2] 孟春春[2] 吴润[1] 刘光清[1,2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《浙江农业学报》2015年第5期734-739,共6页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31270194,31101848,31300141);农业公益性行业科研专项课题(201003012;201303046);国家高科技研究发展计划863计划(2011AA10A209)

摘　　要：应用RT-PCR方法,从北京鸭组织中扩增维甲酸诱导基因I(rerinotic acid inducible gene I,RIG-I),用DNA Star等生物软件分析其所含的生物信息,并构建系统进化树。扩增出的北京鸭RIG-I基因(Gen Bank登录号:KM455977)全长2 802 bp,编码933个氨基酸。RIG-I具有RLR家族的典型特征,即N端含两个半胱天冬酶活化和募集结构域,C端包括RNA解旋酶,ATP结合结构域,RNA结合结构域和抑制结构域,RIG-I蛋白二级结构以无规则卷曲和α-螺旋为主。序列比对和系统进化分析结果表明,RIG-I的序列在同种之间比较保守,与已报道的鸭RIG-I的同源性高达98%以上;但与鹅RIG-I同源性稍低,为95.9%;与人、鼠、草鱼的同源性较低,分别为62.3%,61.5%,61.2%;与猪的同源性最低,仅为54.4%。成功克隆了鸭的RIG-I基因,可为今后开展鸭的天然免疫研究及研发新型疫苗等奠定基础。Duck erinotic acid inducible gene 1 （RIG-Ⅰ） encoding sequence was cloned from spleen of Peking duck, its nucleotide sequence analysis and phylogenetic tree were performed by DNA Star. Nucleotide sequence analysis revealed a sequence of 2 802 bp in length, RIG-Ⅰ encoded a predicted polypeptide of 933 amino acids. RIG-Ⅰ had some typical characteristics of RLR family, for example, there were two caspase activation sites and a recruit domain structure within the N-terminal of RIG-Ⅰ; In the C-terminal of RIG-Ⅰ, the regions of RNA helicase, ATP binding site, RNA binding site and repression domain were also present. The secondary stucture of RIG-Ⅰ showed mainly random coils and alpha helix. Results of phylogenetic tree showed that the sequence of duck RIG-Ⅰ was highly conserved among the same population, which shared 99% homology with the published duck RIG-Ⅰ sequences. But the homology between duck and other species was lower, for example, the identities between duck and goose, homo, mouse, Ctenopharyngodon idella and susscrofa were 95.9% , 62.3% , 61.5% , 61.2% and 54.4% , respectively. In conclusion, duck RIG-Ⅰ gene was successful chined in this study, which would be helpful for the future study of the innate immune of duck and developing new type vaccines.

关 键 词：鸭 RIG-I 克隆 生物信息学分析 进化树 

分 类 号：S852.4[农业科学—基础兽医学] Q785[农业科学—兽医学]