电针对APP/PS1转基因小鼠海马低密度脂蛋白受体相关蛋白-1及其mRNA表达的影响  

Effect of Electroacupuncture on Low Density Lipoprotein Receptor Related Protein-1 and mRNA Expression in APP / PS1 Transgenic Mice Models

作　　者：李芙[1] 王鑫[1] 范盎然[1] 李丽君[1] 加吾拉.阿不力孜 白杨[1] 步青云[1] 高堂珂 李丽娜[1] 薛卫国[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《针灸临床杂志》2015年第5期57-61,F0003,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金资助项目;编号:81273826;北京中医药大学自主选题;编号:2014-JYBZZ-XS-044

摘　　要：目的:研究电针是否是通过影响LRP1mRNA水平、提高LRP1的表达,从而促进脑内Aβ清除。方法:将4月龄APP/PS1转基因鼠,随机分为模型组、电针治疗组,以同窝同背景转基因阴性小鼠为正常对照组。电针干预"涌泉"、"百会"0.1 m A,15 min/次,隔日1次,治疗6周。治疗后,以免疫组化法观察脑组织LRP1阳性表达,以Western blotting法检测海马LRP1表达,以Real-time PCR法检测海马LRP1mRNA表达。结果:LRP1表达于脑微血管内皮细胞、胶质细胞、神经元等处。模型组海马LRP1蛋白、LRP1mRNA相对表达量低于正常对照组(P<0.05),电针治疗组海马LRP1及LRP1mRNA比模型组有上升趋势,但差异无统计学意义(P>0.05)。结论:电针干预可能影响5月龄APP/PS1转基因小鼠海马内所有细胞LRP1及LRP1mRNA表达,可能是电针干预AD发病的潜在靶点,但实验方案还需进一步完善。Objective：To observe whether LRP1 mRNA could be affected by electroacupuncture to improve the expression of LRP1 and strengthen the Aβclearance.Methods：4-month-old Alzheimer＆#39;s disease models of APP/PS1 transgenic mice were randomly divided into a model group（M）and an electro-acupuncture group （EA）.Non-transgenic litter-mate mice were used as a normal control group（C）.Mice in EA group were given acupuncture at “Yongquan”,“Baihui” 0.1 mA, 15 min per time, 1 time every other day,for 6 weeks. After the treatment,immunohistochemistral method was used to observe the LRP1 expression.Western blotting was used to detect LRP1.Real-time PCR was used to detect LRP1mRNA expression in hippocampus.Results：LRP1 immunostaining was positive in microvascular endothelial cells, neurons, glial cells etc.In the model group, LRP1 protein, LRP1mRNA relative expression quantity were lower than those in the normal control group （P〈0.05）.LRP1 and LRP1mRNA in the EA group and the model group had an upward trend, but there was no significant difference （ P 〉0.05 ） .Conclusion：Electroacupuncture may affect all kinds of cells LRP1 and LRP1 mRNA expression in hippocampus of 5-month-old APP/PS1 mice models with Alzheimer＆#39;s disease.It is probable that LRP1 may be a potential target of electroacupuncture intervention on AD.

关 键 词：电针 阿尔兹海默病 Β淀粉样蛋白 低密度脂蛋白受体相关蛋白-1mRNA 

分 类 号：R245.97[医药卫生—针灸推拿学]