刺五加多糖对2、4-二硝基氟苯(DNFB)诱发的超敏反应作用及初步机制研究  

Effect of ASP on Hypersensitivity Induced by DNFB and Its Mechanism

作　　者：张娜[1] 杨晓丹[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2015年第3期26-28,共3页Information on Traditional Chinese Medicine

基　　金：高等学校博士学科点专项科研基金项目(博导类)(No.20132327110009)

摘　　要：目的:研究刺五加多糖(ASP)对免疫功能的调节作用。方法:采用2、4-二硝基氟苯诱导迟发型超敏反应(DTH)模型,采用高、中、低三种浓度ASP对DTH模型小鼠致敏期及诱发期给药,观察小鼠耳组织肿胀、炎性细胞浸润程度,以及胸腺指数、脾脏指数,通过ELISA检测血清中IL-2、IL-4、INF-γ细胞因子的含量。结果:经ASPS给药干预组与DTH模型组耳组织肿胀方面比较有显著性差异(P<0.01),能明显抑制DNFB引起的炎性反应,减少炎性细胞的浸润,降低胸腺和脾脏指数,减少血清中IL-2、IL-4、INF-γ含量。结论:刺五加多糖(ASP)能抑制DTH免疫失衡。Objective：To study the regulative effects of ASP on immune function.Methods：DNFB-induced de-layed type hypersensitivity models were established and high, medium and low ASP concentrations were ad-ministrated during sensitization and induced DTH stage.Evaluate the thymus index and spleen index and ob-serve the swelling of the ear tissues and the degree of inflammatory cell infiltration.Measure the contents of se-rum IL-2,IL-4 and INF-γcytokines by ELISA.Results：The results of ear weighting showed that ASP sig-nificantly inhibited DTH reaction（P〈0.01）.Histological observations indicated ASP markedly reduced the infiltration of lymphocytes.ASP could increase the thymus index and spleen index,and the contents of IL-2, IL-4 and INF-γ.Conclusion：ASP can significantly improve the cellular immune function.

关 键 词：刺五加多糖 免疫功能 DTH 

分 类 号：R285.5[医药卫生—中药学]