不同激素对雌性花鳗鲡性体指数和发育过程中促性腺激素基因(GtHα,FSHβ,LHβ)表达的影响  

Impact of injection of different hormones on gonadosomatic index and expression of gonadotropin genes( Gt Hα,FSHβ,LHβ) in female marbled eel( Anguilla marmorata)

作　　者：王磊[1] 李响[2] 柳峰松[2] 刘利平[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]河北大学生命科学学院,河北保定071002

出　　处：《水产学报》2015年第5期609-617,共9页Journal of Fisheries of China

基　　金：上海市农委科技攻关项目(沪农科攻字2013第2-2号);上海市科学技术委员会项目(13320502200,08391910200)

摘　　要：为评估不同激素诱导花鳗鲡卵巢发育的效果并探究促性腺激素在花鳗鲡发育过程中的作用,实验比较了不同激素组合处理后花鳗鲡性体指数（gonadosomatic index,GSI）的变化,并采用实时荧光定量PCR（qRT-PCR）对处于不同发育阶段雌鱼脑垂体中促性腺激素基因GtHα（促性腺激素亚基）,LHβ（促黄体生成素亚基）及FSHβ（卵泡刺激素亚基）的表达进行了定量研究。结果发现,鲤脑垂体萃取液（CPE）与人类绒毛膜促性腺激素（hCG）组合能够有效促使雌性花鳗鲡性腺发育成熟,GSI平均达22.9%,与对照组相比差异显著。单独注射雄烯二酮（ADSD）和注射ADSD、CPE、hCG混合制剂诱导效果不明显,GSI仅为1%-3%。荧光定量发现,经CPE与hCG处理的亲鱼LHβ和GtHα表达量不断上调,其中发育早期GtHα表达量极显著升高,而FSHβ的表达量呈现下调趋势。研究表明,CPE与hCG组合注射能够成功诱导花鳗鲡卵巢发育成熟,而ADSD对花鳗鲡卵巢发育无明显促进作用。LH在花鳗鲡卵巢发育整个过程中扮演重要角色;FSH在花鳗鲡性腺发育早期作用显著,可能是诱导鳗鲡性腺早期发育的关键因素。In order to study the effect of different hormones injection on marbled eel Anguilla marmorata ovarian development and to clarify the role of two gonadotropins in the process,this paper compares the induction effects treated by different hormone combinations through GSI（ Gonadosomatic index）,and a quantitative study by real-time fluorescent quantitative PCR（ qRT-PCR） was applied to analyze the expression of the pituitary gonadotropin hormone subunits： GtHα,LHβ and FSHβ genes at different developmental ovary stages. The results show ed that combination of CPE（ carp pituitary extract） and hCG（ human chorionic gonadotropin） can effectively induce the gonadal maturation of eels with GSI significantly increased. How ever,injection of androstenedione（ ADSD） alone and mixture of ADSD,CPE and hCG didn＇t have significant impact on GSI w hich varied w ithin 1%- 3%. The results of quantitative fluorescence PCR show ed that expression of LHβ and G t Hα significantly increased among the CPE and hCG treated group,w hile the expression quantity of FSHβ w as dow n-regulated. In conclusion,CPE and hCG could successfully induce female maturation,w hile injection of ADSD into female eel had no induction of gonadal development; meanw hile,LH plays a vital role in gonadal development in A. marmorata,and FSH plays a key role in early gonadal development,indicating that the FSH may be the key factor inducing the early development of eel＇s ovary.

关 键 词：花鳗鲡 性腺发育 卵泡刺激素 黄体生成素 雄烯二酮 

分 类 号：Q785[生物学—分子生物学] S965[农业科学—水产养殖]