不同溶氧变动模式对鲻生长、能量代谢和氧化应激的影响  

Effect of variation of different dissolved oxygen on the growth,energy metabolism and oxidative stress of Mugil cephalus

作　　者：刘旭佳[1] 黄国强[1] 彭银辉[1] 

机构地区：[1]广西海洋研究所海洋生物技术重点实验室,广西北海536000

出　　处：《水产学报》2015年第5期679-690,共12页Journal of Fisheries of China

基　　金：广西自然科学基金(2011GXNSFA018116)

摘　　要：为研究不同溶氧变动模式对鲻生长、能量代谢和氧化应激的影响,实验设计5种溶氧变动模式,分别为一直维持正常溶氧N处理(7.0 mg/L)、正常溶氧-低氧变动N-L处理(7.0→1.5 mg/L)、超饱和溶氧-正常溶氧变动S-N处理(14.0→1.5 mg/L)、超饱和溶氧-正常溶氧-低氧变动S-N-L处理(14.0→7.0→1.5 mg/L)和一直维持超饱和溶氧S处理(14.0 mg/L)。实验选择初始体质量为(16.07±0.11)g的鲻,在循环水装置中养殖56 d,然后测定其特定生长率、鲻血浆、肌肉、肝脏和鳃组织的乳酸(LD)含量、过氧化物歧化酶(SOD)活力、总抗氧化能力(T-AOC)、抗超氧阴离子活力(ASOR)、丙二醛(MDA)含量、总谷胱甘肽(T-GSH)、氧化型谷胱甘肽(GSSG)和还原型谷胱甘肽的含量。测定终末体质量为(31.47±1.44)g鲻的耗氧率、排氨率和氧氮比。结果显示,5种溶氧变动模式对鲻生长影响显著,N、S和S-N处理的鲻特定生长率显著高于N-L和S-N-L处理。S处理的鲻排氨率和氧氮比显著高于其他处理。5种溶氧变动模式均对鲻氧化应激指标含量影响显著。研究表明,肝脏是主要氧化应激器官。经历低氧变动模式的鲻,代谢速率下降,同时需要消耗较多的物质和能量参与氧化应激,用于生长物质和能量需求减少从而导致生长速度下降。T-GSH含量与氧化压力呈负相关,在应对低氧和超饱和溶氧产生的氧化压力中起重要调节作用。Dissolved oxygen is one of the most important factors affecting the fish grow th and metabolism.Some fish exposition to hypoxia and super-saturated dissolved oxygen may result in oxidative stress evidenced by variation in activities of antioxidant enzymes. Mugil cephalus has higher economic value,and it has not been reported that there are the effects of different dissolved oxygen variation on the grow th,energy metabolism and oxidative stress. In order to find the relationship and consequences,experiments were carried out according to experimental ecology,bioenergetics and physiology methods. The experiments w ere conducted under five modes of variation of different dissolved oxygen,i. e. N（ 7. 0 mg / L）,N-L（ 7. 0→1. 5mg / L）,S-N（ 14. 0 → 1. 5 mg / L）,S-N-L（ 14. 0 → 7. 0 → 1. 5 mg / L）,and S（ 14. 0 mg / L） treatments,respectively. The healthful individuals（ 16. 07 ± 0. 11） g in mean initial total w eight w ere cultivated for 56 days in the cycling w ater installation. The specific grow th rate,oxidative stress indicators were measured including the content of LD,SOD,T-AOC,ASOR,M DA,T-GSH,GSSG,and GSH in the plasma,muscle,liver and gill. Then the healthful individuals（ 31. 47 ± 1. 44） g in mean final total weight were selected and the oxygen consumption rate,ammonia excretion rate,O ： N ratios were measured. The results show ed that five modes of variation of different dissolved oxygen had a significant effect on the grow th of mullet. The SGR of mullet in the N,S and S-N treatments were significantly higher than in N-L and S-N-L treatments.The ammonia excretion rate and O ： N ratios in S treatment was the highest compared to the other treatments.Five modes had a great influence on the oxidative stress indicators. The study found out that liver was the most important organ to respond to the oxidative stress. The metabolic rate decreased in the modes containing the hypoxia stage,moreover,the mullets need to consume more material and energy involved in the oxidative stress

关 键 词：鲻 溶氧变动 能量代谢 氧化应激 

分 类 号：S917.3[农业科学—水产科学]