葛根素预处理对局灶性脑缺血再灌注大鼠海马CA1区神经元损伤的影响及其机制  

Effects and related mechanisms of preconditioning with puerarin on hippocampus CA1 region neuronal injury after focal cerebral ischemia-reperfusion

作　　者：尹雪莉[1] 桂丽[2] 李珍[1] 

机构地区：[1]安徽医科大学基础医学院生理学教研室,合肥230032 [2]安徽医科大学基础医学院综合实验室,合肥230032

出　　处：《安徽医科大学学报》2015年第6期723-726,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30900420);安徽医科大学校科学研究基金(编号:2012xkj007)

摘　　要：目的探讨葛根素预处理对局灶性脑缺血再灌注大鼠海马CA1区神经元损伤的保护作用及其机制。方法雄性SD大鼠30只,随机均等分为假手术组、模型组、葛根素预处理组。采用线栓法阻断大鼠大脑中动脉血供,60 min后拔出栓线造成局部脑缺血再灌注损伤。葛根素预处理组在缺血前1 h腹腔注射葛根素(100 mg/kg)。脑缺血再灌注后第5天,HE染色法观察海马CA1区神经元的组织形态学变化,免疫组化法检测海马CA1区半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)及半胱氨酸天冬氨酸蛋白酶-9(Caspase-9)的表达。结果 HE染色结果显示,与模型组相比较,葛根素预处理明显减少脑缺血再灌注引起的海马CA1区神经元损伤(P<0.05);免疫组化结果显示,与模型组相比,葛根素预处理使海马CA1区Caspase-3、Caspase-9的表达明显降低(P<0.05)。结论缺血前1 h葛根素预处理对局灶性脑缺血大鼠海马CA1区神经元损伤有保护作用,其主要机制可能与抑制凋亡相关蛋白Caspase-3、Caspase-9的表达有关。Objective To observe the effect of puerarin preconditioning on hippocampal CA1 region neuronal injury and further explore its mechanisms. Methods 30 male Sprague-Dawley rats were randomly assigned into sham, model,and puerarin preconditioning ischemia-reperfusion groups. The middle cerebral artery was occluded for 60 min by an intraluminal filament before reperfusion to induce transient focal cerebral ischemia reperfusion rats. Puer-arin preconditioning groups rats received puerarin（100 mg / kg,i. p）1 h before ischemia. The histological changes of hippocampal CA1 region neurons were measured by HE staining on the 5th day after reperfusion. The expressions of Caspase-3 and Caspase-9 proteins in the CA1 region were examined by immunohistochemistry staining. Results The results of HE staining showed that,compared with the model group,puerarin preconditioning significantly re-duced the number of cerebral ischemia induced injury of hippocampal CA1 region neurons（P 〈 0. 05）. Immunohis-tochemistry data showed that the expressions of Caspase-3 and Caspase-9 in the hippocampal CA1 region of puerarin preconditioning group were significantly lower than that of model group（P 〈 0. 05）. Conclusion The results indi-cate that puerarin preconditioning 1 h before ischemia exerts neuroprotective effects on focal cerebral ischemia-reperfusion induced neuronal injury. The neuroprotective mechanisms of puerarin may attribute to inhibiting the ex-pressions of Caspase-3 and Caspase-9 protein.

关 键 词：葛根素 缺血再灌注损伤 神经保护 Caspase-3 CASPASE-9 

分 类 号：R33-33[医药卫生—人体生理学] R932[医药卫生—基础医学]