双酚A对小鼠腹腔巨噬细胞极化影响的体外研究  

Effects of bisphenol A on polarization of mice peritoneal macrophage in vitro

作　　者：李美玲[1] 李应配 冷银芝 蒋建华[2] 朱启星[3] 沈彤[1,3] 

机构地区：[1]安徽医科大学公共卫生学院卫生毒理学系,合肥230032 [2]安徽医科大学第一附属医院临床营养科,合肥230022 [3]安徽医科大学公共卫生学院职业卫生与环境卫生学系,合肥230032

出　　处：《安徽医科大学学报》2015年第6期782-786,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81473015)

摘　　要：目的探讨体外实验条件下,双酚A(BPA)对小鼠腹腔巨噬细胞极化的影响。方法提取C57BL/6J雄性小鼠腹腔巨噬细胞,用(10 ng/ml)干扰素-γ(IFN-γ)和(500 ng/ml)细菌脂多糖(LPS)诱导其向M1型极化;用(10 ng/ml)白介素-4(IL-4)诱导其向M2型极化;同时加入0.1、1、10μmol/L BPA处理细胞,并设立空白对照组和溶剂对照组。流式细胞术检测M1型和M2型巨噬细胞比例,ELISA法检测M1型和M2型巨噬细胞各自分泌的诱导型一氧化氮合酶(i NOS)和精氨酸酶(Arg-1)活性。结果不同浓度BPA促进IFN-γ和LPS诱导的小鼠腹腔巨噬细胞向M1型极化,1、10μmol/L BPA组M1型巨噬细胞比例较空白对照组分别升高11.3%和17.4%,M1型巨噬细胞分泌的i NOS活性分别升高1.95和2.29倍,差异均有统计学意义。不同浓度BPA抑制IL-4诱导的小鼠腹腔巨噬细胞向M2型极化,1、10μmol/L BPA组M2型巨噬细胞比例分别较空白对照组降低9.0%和14.3%,M2型巨噬细胞分泌的Arg-1活性分别下降0.37和0.49倍,差异均有统计学意义。结论体外条件下,低剂量BPA促进小鼠腹腔巨噬细胞向M1型极化,抑制其向M2型极化。Objective To explore the effect of bisphenol A（BPA）on the polarization of mice peritoneal macro-phage in vitro. Methods Peritoneal macrophages isolated from C57BL/ 6J male mice were stimulated with murine （10 ng / ml）IFN-γ and LPS（500 ng / ml）for induction of M1-type macrophages,and（10 ng / ml）IL-4 for M2-type,respectively. Meanwhile cells were treated with 0. 1,1 and 10 μmol/ L BPA,blank control group and vehicle control group were established at the same time. The proportions of M1-type and M2-type macrophages were deter-mined by FACS analysis,the activities of iNOS（cytokines secreted by M1）and Arg-1（cytokines secreted by M2） were measured by ELISA assay. Results Different concentrations of BPA promoted M1-type polarization of mice peritoneal macrophage induced by IFN-γ and LPS. Compared with the blank control group,M1 type proportion was increased by 11. 3% and 17. 4% ,and iNOS activity was elevated by 1. 95 and 2. 29 times in 1 and 10 μmol/ L BPA group respectively,and the differences were statistically significant. Different concentrations of BPA inhibited M2-type polarization of mice peritoneal macrophage induced by IL-4. Compared with the blank control group,M2 type proportion was decreased by 9% and 14. 3% and Arg-1 activity was reduced by 0. 37 and 0. 49 times in 1 and 10 μmol/ L BPA group respectively,and the differences were statistically significant. Conclusion Low dose expo-sure of BPA can promote M1-type polarization of mice peritoneal macrophage,while inhibit M2-type polarization in vitro.

关 键 词：双酚 A 巨噬细胞 极化 

分 类 号：R114[医药卫生—卫生毒理学]