冠心病患者血清可溶性纤维介素2的变化及其与炎症因子的关系  

Changes of serum s FGL2 level and its relationship with inflammation cytokines in patients with coronary heart disease

作　　者：汪道利 何非[1] 许邦龙[1] 吴继雄[1] 王晓晨[1] 盛建龙[1] 

机构地区：[1]安徽医科大学第二附属医院心血管内科,合肥230601

出　　处：《安徽医科大学学报》2015年第6期816-820,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽医科大学校科学研究基金(编号:2011xkj058)

摘　　要：目的探讨冠心病患者外周血清中可溶性分泌型纤维介素2(s FGL2)水平及其与炎症因子的关系。方法选择52例初诊急性冠脉综合征(ACS)患者、50例初诊稳定性心绞痛(SAP)患者和50例冠脉造影正常者作为研究对象,分别测定外周血CD4+CD25+CD127low调节性T淋巴细胞(Treg)、外周血s FGL2、炎症因子白细胞介素-10(IL-10)、转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)和超敏C反应蛋白(hs-CRP)水平,RT-PCR测定外周血淋巴细胞膜型纤维介素2(FGL2)mRNA表达量,并进行统计学分析。结果与对照组和SAP组相比,ACS组外周血CD4+CD25+CD127lowTreg百分比及血清s FGL2、IL-10、TGF-β1显著降低(P<0.01),而TNF-α和hs-CRP显著升高(P<0.01)。血清s FGL2与外周血Treg、IL-10、TGF-β1呈正相关性,与TNF-α和hs-CRP呈负相关性。逐步多元回归分析表明,s FGL2与外周血Treg和IL-10呈正相关性。结论 ACS患者外周血s FGL2明显降低,可以反映循环血CD4+CD25+CD127lowTreg数量及血清IL-10水平,s FGL2降低可能参与了ACS发生和发展。Objective To investigate the serum level of soluble fibrinogen-like protein 2（sFGL2）and its relation-ship with inflammation cytokines in the patients with coronary artery disease. Methods 152 consecutive patients were enrolled in this study. Patients were divided into acute coronary syndrome（ACS）group,stable angina pecto-ris（SAP）group and control group according to coronary angiography. Flow cytometry was employed to detect num-ber of circulating CD4 ＋ CD25 ＋ CD127low Treg cells. Serum concentrations of sFGL2 and inflammation cytokines in-cluding IL-10,TGF-β1,and hs-CRP were measured by ELISA. FGL2 mRNA in peripheral blood mononuclear cells （PBMCs）obtained from these patients was measured by RT-PCR. The association of sFGL2 with circulating CD4 ＋CD25 ＋ CD127low Treg and inflammation cytokines was analyzed. Results Compared with the control and SAP group,the concentrations of sFGL2 were significantly lower（P 〈 0. 01）,while concentrations of IL-10 and TGF-β1 significantly decreased（P 〈 0. 01）. TNF-α and hs-CRP significantly elevated in ACS group（P 〈 0. 01）. FGL2 mRNA measured by RT-PCR indicated that expression of FGL2 significantly reduced in ACS group compared with SAP and the control group. Serum concentration of sFGL2 was positively correlated with circulating CD4 ＋ CD25 ＋CD127low Treg level,IL-10 and TGF-β1 while negatively correlated with TNF-α and hs-CRP. Stepwise multi-re-gression analysis showed that serum sFGL2 level was significantly associated with circulating CD4 ＋ CD25 ＋ CD127low Treg and IL-10 level. Conclusion Decreased concentration of sFGL2 is an independent risk factor for ACS. Changes in sFGL2 concentration can reflect the levels of circulating CD4 ＋ CD25 ＋ CD127low Treg and IL-10.

关 键 词：冠心病 急性冠脉综合征 可溶性纤维介素2 炎症因子 

分 类 号：R541.4[医药卫生—心血管疾病]