纯镁微弧氧化-HF-硅溶胶复合处理涂层细胞相容性研究  

Biocompatibility of Pure Magnesium Ultrasonic Micro-arc Oxidation-HF-Silica Sol Compounded Treatment

作　　者：刘继光[1] 孙玉龙[1] 李慕勤[1] 刘江[1] 李晓光[1] 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《口腔医学研究》2015年第5期429-432,436,共5页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:31370979);黑龙江省高校创新团队建设计划项目(编号:2012TD010)

摘　　要：目的:对纯镁微弧氧化经HF-硅溶胶复合处理涂层进行成骨细胞生物相容性检测,评价纯镁表面微弧氧化经不同复合处理涂层后的细胞相容性。方法:实验分为3组,纯镁微弧氧化组为对照组(A组)、纯镁微弧氧化-硅溶胶复合处理组(B组)、纯镁微弧氧化-HF-硅溶胶复合处理组(C组)。将MC3T3-E1系成骨细胞接种在3组材料表面,分别通过扫描电镜、激光共聚焦、CCK-8、ALP对成骨细胞的生长、黏附、增殖以及活性进行检测。结果:扫描电镜和激光共聚焦显示B组和C组表面细胞的生长和黏附均优于A组,C组优于B组。CCK-8和ALP的检测结果显示3组材料表面细胞的增殖、活性为C组>B组>A组,3组的比较差异均具有统计学意义。结论:3组材料均具有良好的生物相容性,而纯镁微弧氧化-HF-硅溶胶复合处理后的生物相容性最佳。Objective：To study the biocompatibility of pure magnesium micro-arc oxidation- HF-silica sol composite treatment,and to evaluate the biocompatibility of pure magnesium micro-arc oxidation after different composite treatment.Methods：The experiment was divided into three groups,the pure magnesium micro-arc oxidation was used as the control group（group A）.The pure magnesium micro-arc oxidation-silica sol composite treatment was group（group B）.The pure magnesium micro-arc oxidation-HF-silica sol composite treatment was group（group C）.MC3T3-E1 osteoblasts were inoculated on material surface of three groups,by scanning electron microscopy（SEM）,laser confocal scanning microscope,CCK-8,ALP to test respectively growth,adhesion,proliferation and activity of osteoblasts.Results：The scanning electron microscope and laser confocal scanning microscope demonstrated that on cell growth and adhesion group B and group C surface were superior to group A.Group C was better than that of group B.The results of CCK-8and ALP detection showed that the proliferation and activity of material surface of three groups were group C 〉 group B 〉 group A.The comparative differences of three groups were statistically significant.Conclusion：The pure magnesium micro-arc oxidation silicon sol composite treatment and pure magnesium micro-arc oxidation-HF-silicon sol composite treatment had all good biocompatibility,and the biocompatibility of HF-silica sol composite treatment was the best.

关 键 词：纯镁 微弧氧化 HF 硅溶胶 成骨细胞 

分 类 号：R318.08[医药卫生—生物医学工程]