3种义齿粘附剂的体外细胞毒性研究  

In Vitro Studies on the Cytotoxicity of Three Commercial Denture Adhesives

作　　者：陈凤英[1] 程祥荣[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2015年第5期457-460,共4页Journal of Oral Science Research

摘　　要：目的：体外研究3种商品化义齿粘附剂的细胞毒性。方法：采用MTT法检测不同浓度义齿粘附剂浸提液对人原代口腔黏膜角质细胞、成纤维细胞和小鼠成纤维细胞系L929的细胞毒性。结果：3种义齿粘附剂对人原代口腔黏膜角质细胞有1~2级细胞毒性,对人原代口腔黏膜成纤维细胞有1级细胞毒性,对L929细胞却无毒性。高浓度义齿粘附剂浸提液一般比低浓度浸提液具有更强的细胞生长抑制作用。结论：义齿粘附剂对人原代口腔黏膜角质细胞和成纤维细胞有细胞毒性,对L929细胞却无细胞毒性,提示在义齿粘附剂的细胞毒性测试方面,人原代口腔黏膜细胞可能会提供更有价值的信息。Objective：To investigate the cytotoxicity of three commercial denture adhesives.Methods：The cytotoxic effects of three denture adhesive eluates with different concentrations on primary human oral keratinocytes（HOKs）,fibroblasts（HOFs）and permanent mouse fibroblasts cell lines（L929）were tested using MTT assay.Results：Three tested denture adhesives showed cytotoxicity to primary HOKs（levels 1to 2）and HOFs（level 1）,whereas none of three was toxic to L929 cells.Higher concentrations of adhesive eluates generally showed significantly stronger inhibitory effects than the lower ones.Conclusion：Denture adhesives are toxic to the primary HOKs and HOFs cultures,whereas non-toxic to L929 cells.The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives.

关 键 词：义齿粘附剂 人原代口腔黏膜细胞 MTT法 细胞毒性 

分 类 号：R783.1[医药卫生—口腔医学]