氢盐水对百草枯中毒小鼠急性肺损伤及肺组织血红素氧化酶-1表达的影响  

Effect of hydrogen-saline on lung injury and heme oxygenase-1 expression in the lung tissue of acute paraquat-intoxicated mice

作　　者：刘刚[1] 宋冬梅[2] 江宇[3] 葛赟 

机构地区：[1]重庆医科大学附属大学城医院急诊科,401331 [2]河北医科大学第一医院耳鼻喉科实验室 [3]重庆医科大学附属大学城医院心脏呼吸中心 [4]杭州市滨江医院急诊科

出　　处：《中华劳动卫生职业病杂志》2015年第5期337-341,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的 探讨氢盐水对百草枯（PQ）中毒小鼠急性肺损伤以及对肺组织血红素氧化酶-1（HO-1）表达的影响及机制.方法 108只雄性清洁级ICR小鼠随机分为对照组（8只）,PQ组（50只）和PQ＋氢盐水组（50只）,PQ组质量浓度为20％的PQ 50 mg/kg一次性灌胃.PQ＋氢盐水组20％的PQ50 mg/kg一次性灌胃后予饱和氢气生理盐水5 ml/kg腹腔注射,2次/d.观察行为学变化,不同处理后6h、1、3、7、14d测定肺组织匀浆丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力,HE染色观察肺组织损伤情况,免疫印迹法和免疫组化法测定肺组织HO-1含量.结果 PQ中毒后小鼠出现活动减少,呼吸急促甚至死亡等表现,PQ＋氢盐水组比PQ组有所减轻.PQ组6h及24 h肺组织匀浆MDA含量明显高于对照组,PQ组6、24h及3、7d时的SOD活力明显低于对照组,差异有统计学意义（P＜0.05）.PQ＋氢盐水组24 h MDA含量明显低于PQ组,24 h及3、7d时PQ＋氢盐水组SOD活力明显高于PQ组,差异有统计学意义（P＜0.05）.PQ＋氢盐水组HO-1表达趋势及分布部位与PQ组相似,但各时间点HO-1表达HIS值均明显高于PQ组和对照组,差异均有统计学意义（P＜0.05）.结论 氢盐水可上调PQ中毒小鼠肺组织HO-1的表达,有减轻PQ中毒肺损伤的效应.Objective To investigate the effect of Hydrogen-saline on Lung Injury and HO-1 Expression in The Lung Tissue of Acute Paraquat-intoxicated mice.Methods 108 male clean-grade mice were divided randomly into 3 groups：normal control group（n=8）,PQ group（n=50） and PQ HN group（n=50）,PQ group were lavaged with 20％ PQ（50 mg/kg）.PQ＋Hyclrogen saline group were intxaperitoneal injected with 5 ml/kg saturated hydragen saline after lavaged,2 times/d.We observe its performance after the poisoning,The lung tissue were taken to HE stained,MDA and SOD activity of lung tissue homogenate were detected,HO-1 activity were observed by immunohistochemistry and western method at 6 h,1 d,3 d,7 d and 14 d.Results mice came to shortness of breath,exhaustion and death after poisoned.PQ＋hydrogen saline group was more alleviative than PQ group.The lung MDA of PQ group was markedly increased at 6 h and 24 h and SOD was decreased at 6 h,24 h,3 d,7 d,14 d than normal control group.In Comparison with PQ group,the lung MDA was decreased at 24 h and SOD was increased at 24 h and 3 d （P＜0.05）.HO-1 expression trend and distribution in PQ＋hydrogen saline group are similar with PQ group,but were significantly higher than that of PQ group and the control group each time point （P＜0.05）.Conclusion Oxidative stress plays important roles in lung injury caused by paraquat.Hydrogen-saline may increase expression of HO-1 and alleviate oxidative stress damage in lung.

关 键 词：氢盐水 百草枯 急性呼吸窘迫综合征 血红素氧化酶(脱环) 

分 类 号：R965[医药卫生—药理学]