山东不同地区韭菜迟眼蕈蚊共生菌Wolbachia的检测及鉴定  

Detection and identification of Wolbachia in Bradysia odoriphaga( Diptera: Sciaridae) populations from Shandong Province,China

作　　者：陶云荔[1] 郭雅男 王静[2] 李丽莉[2] 于毅[2] 褚栋[1] 

机构地区：[1]青岛农业大学农学与植物保护学院山东省植物病虫害综合防控重点实验室,山东青岛266109 [2]山东省农业科学院植物保护研究所山东省植物病毒学重点实验室,济南250100

出　　处：《昆虫学报》2015年第4期454-459,共6页Acta Entomologica Sinica

基　　金：国家公益性行业(农业)科研专项(201303027);山东省现代农业产业技术体系

摘　　要：【目的】为了揭示山东省韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang种群共生菌Wolbachia的感染率及其分类地位,探讨该共生菌对韭菜迟眼蕈蚊的潜在影响。【方法】利用线粒体细胞色素氧化酶I（mt COI）基因引物（LCO1490/HCO2198）,通过扩增测序和序列比对对采自山东省12个地区的根蛆种群进行了分类鉴定。在上述基础上,利用Wolbachia的16S r DNA和wsp基因特异引物（分别为16S-F/16S-R和81F/691R）对鉴别出的11个韭菜迟眼蕈蚊种群体内Wolbachia感染情况进行了PCR检测;对感染个体体内Wolbachia依据16S r DNA基因片段序列进行分类鉴定。【结果】山东省12个根蛆种群中,11个种群为韭菜迟眼蕈蚊种群。基于Wolbachia的16S r DNA基因特异引物检测结果发现,这些韭菜迟眼蕈蚊种群广泛感染Wolbachia（感染率为6.67%~93.33%）,而利用wsp基因特异引物检测的感染率（0.00%~40.00%）相对较低些。基于Wolbachia的16S r DNA基因构建系统发育树表明,这些韭菜迟眼蕈蚊种群感染的Wolbachia全部属于A组。【结论】确定了Wolbachia在山东省韭菜迟眼蕈蚊体内的感染率及其分类地位,为研究Wolbachia对韭菜迟眼蕈蚊生物学及生态学的影响奠定了基础。【Aim 】 This study aims to determine the infection rate and taxonomic identity of the endosymbiont Wolbachia in the populations of Bradysia odoriphaga Yang et Zhang in Shandong Province,so as to reveal the potential effects of this endosymbiont on B. odoriphaga. 【Methods 】The species identity of 12 chive maggot populations collected in Shandong was first identified based on the mitochondrial cytochrome oxidase I（ mt COI） gene. Two pairs of primers（ 16S-F /16S-R and 81 F /691R）were used to detect the infection of Wolbachia in the 11 populations identified as B. odoriphaga. The Wolbachia infecting these B. odoriphaga populations were further identified based on the 16 S r DNA sequence. 【Results】Compared with the infection rates of Wolbachia（ ranging from 6. 67% to 93. 33%）based on 16S-F /16S-R primers, the infection rate of Wolbachia based on 81 F /691 R primers was relatively low（ 0. 00%- 40. 00%）. Phylogenetic tree based on the 16 S r DNA sequence showed that all of the Wolbachia detected belonged to group A. 【Conclusion】This study reveals the infection rate of Wolbachia in B. odoriphaga in Shandong and their taxonomic position,and these results provide the basis for future study on the effects of Wolbachia on the biology and ecology of B. odoriphaga.

关 键 词：韭菜迟眼蕈蚊 分子鉴定 WOLBACHIA 16S RDNA WSP 山东 

分 类 号：Q965.9[生物学—昆虫学]