纳米银对金黄色葡萄球菌的抗菌作用及其机制研究  

Antibacterial effect of silver nanoparticles on Staphylococcus aureus and the underlying mechanism

作　　者：段晓杰[1] 杜晓丹[1] 张蓓蓓[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《生物医学工程与临床》2015年第3期237-240,共4页Biomedical Engineering and Clinical Medicine

摘　　要：目的以金黄色葡萄球菌为对象,研究纳米银的抗菌作用,并对抗菌机制进行探讨。方法金黄色葡萄球菌(ATCC6538)、双倍浓度营养肉汤和营养肉汤由中国食品药品检定研究院提供;纳米银粉末(≤100 nm,576832-5G,SIGMA-ALORICH)。采用最小抑菌浓度(MIC)实验,测定纳米银对金黄色葡萄球菌的振荡培养时(转速为300 r/min)最小抑菌浓度值;采取烧瓶振荡法测定质量浓度50.00、100.00、200.00、800.00μg/m L纳米银对金黄色葡萄球菌作用的光密度(OD)值;扫描电子显微镜观察纳米银处理后金黄色葡萄球菌结构的变化。结果振荡培养时,纳米银对金黄色葡萄球菌的MIC值为800.00μg/m L;纳米银使金黄色葡萄球菌的延滞期延长,浓度800.00μg/m L纳米银溶液能够完全抑制金黄色葡萄球菌在肉汤培养液中的增殖生长;用50.00μg/m L的纳米银作用金黄色葡萄球菌4 h,电子显微镜结果显示,金黄色葡萄球菌细胞壁破裂、胞内物质外流及细胞的死亡。结论纳米银对金黄色葡萄球菌有抗菌作用,可能进入细菌细胞内造成其死亡。Objective To study the antibacterial activity of nanosilver on Staphylococcus aureus and the underlying mechanism.Methods The Staphylococcus aureus（ATCC6538）, double strength nutrient broth and nutrient broth were provided by National Institute for Food and Drug Control. The diameter of silver powder was less than 100 nm（576832-5G, SIGMA-ALORICH）. The minimal inhibitory concentration（MIC） of silver nanoparticles was determined against Staphylococcus aureus in culture at rotation speed of 300 r/min, and shaking-flask method was used to measure the optical density（OD） value of Staphylococcus aureus suspension at 50.00, 100.00, 200.00, and 800.00 μg/m L. The structure of Staphylcoccus aureus treated with silver nanoparticles was observed by scanning electric microscopy. Results The results showed that MIC of silver nanoparticles against Staphylococcus aureus was 800.00 μg/m L. The silver nanoparticles at 800.00 μg/m L inhibited the growth of bacteria in nutrient broth completely.The growth curves showed that silver nanoparticles prolonged the lap phase of Staphylcoccus aureus. After exposure to 50.00 μg/m L silver nanoparticles for 4 hours, it was observed that the cell wall was broken and the inner material flowed out, indicatied the death of cells. Conclusion It is demonstrated that silver nanoparticles have antibacterial activity on Staphylcoccus aureus, which could get into the cell and lead to cell death.

关 键 词：纳米银 金黄色葡萄球菌 抗菌作用 

分 类 号：R96[医药卫生—药理学]