慢性给予GHRP-6对小鼠跑轮运动日节律的影响  

Effects of Chronic GHRP-6 Administration on the Circadian Wheel-running Rhythm in Mice

作　　者：郝维[1] 周岚[1] 谷婧丽[1] 曹济民[1] 

机构地区：[1]中国医学科学院基础医学研究所/北京协和医学院基础学院生理学系,100005

出　　处：《医学研究杂志》2015年第5期24-27,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81071072;31471126)

摘　　要：目的本研究探讨慢性给予GHRP-6对小鼠日运动节律的影响。方法利用Mini Mitter鼠类跑轮活动监测系统实时记录C57BL6/J小鼠在全黑暗条件下跑轮运动的昼夜节律;在不同时间点(CT6、CT12、CT14、CT18、CT22和CT24)每日腹腔注射GHRP-6(100μg/kg),观察GHRP-6对小鼠跑轮运动昼夜节律和运动活性的影响。结果在CT12给予GHRP-6引起明显的跑轮运动日节律时相延迟,而在其他时间点不引起明显的时相位移。GHRP-6同时引起一定程度的运动活性降低,但该效应与给药时间没有明显关系。结论 GHRP-6可引起C57BL6/J小鼠的跑轮运动日节律时相的时间依赖性延迟和时间非依赖性运动活性降低,该研究提示GHSR信号通路对生物钟有调节作用。Objective To investigate the effect of GHRP -6 on the circadian locomotor rhythm of mice. Methods The circadian wheel -running activities of C57BL6/J mice were monitored with the MiniMitter Data Acquisition System under constant darkness （DD）. GHRP - 6 （100μg/kg） was injected （ip） daily for 14 days at different circadian times （ CT6, CT12, CT14, CT18, CT22 and CT24）. Re- suits Repeated GHRP - 6 injection at CT12 induced significant phase delay of the circadian rhythm, but not at other CTs. Repeated GH- RP - 6 injection also suppressed the activity intensities, but this effect was not strictly time - dependent. Conclusion GHRP - 6 can in- duce time - dependent phase delay of the circadian rhythms and time - independent suppression of activity intensities in mice. The study suggests that GHSR signaling has a regulatory role in the circadian rhythm.

关 键 词：生长激素释放肽 生长激素促泌素受体昼夜节律 相移 生物钟 

分 类 号：R3[医药卫生—基础医学]