人参皂甙Rd对结核感染后中性粒细胞的保护作用  

Ginsenoside Rd Protects the Infected with Mtb of Neutrophils

作　　者：王雯菁 冯国栋[1] 赵青[1] 朱旗[1] 张敏[1] 刘扬[1] 史明[1] 赵钢[1] 

机构地区：[1]第四军医大学西京医院神经内科,西安710032

出　　处：《医学研究杂志》2015年第5期32-36,共5页Journal of Medical Research

摘　　要：目的研究人参皂甙Rd(ginsenoside Rd)对健康成年人外周血、结核感染模型的中性粒细胞(PMN)凋亡率的影响。方法提取健康成年人外周血中PMN,分为3组:1组:PMN与人参皂甙Rd共培养;2组:PMN与人型结核分枝杆菌减毒株(H37Ra)和人参皂甙Rd共培养;3组:PMN与人体皮下注射用卡介苗(BCG)和人参皂甙Rd共培养。各组对照组分别加入等体积的丙二醇。3组PMN分别培养24、48h,采用流式细胞术检测PMN凋亡率。结果各组培养24h后,人参皂甙Rd浓度为100μmol/L时PMN凋亡率较对照组明显降低(P<0.05);培养48h后,1组与3组PMN于人参皂甙Rd浓度为100μmol/L时凋亡率较对照组显著降低(P<0.05);与2组比较差异无统计学意义。结论高浓度的人参皂甙Rd能够抑制正常中性粒细胞和结核感染模型中性粒细胞的凋亡,提高细胞存活率,延长细胞存活时间。Objective To study the influence of Ginsenoside Rd on the apoptosis rate of healthy adults＇ peripheral blood and the PMN in TB infection model. Methods As for the methods, the PMN of healthy adults peripheral blood were taken and divided into three groups. The first group was the co - cultivation of PMN and Ginsenoside Rd. The second group was the co - cultivation of PMN, mycobac- terium tuberculosis attenuated strain （H37Ra） and Ginsenoside Rd. The third group was the co - cultivation of PMN, human subcutane- ous injection bacillus Calmette Guerin （BCG） and Ginsenoside Rd. All control groups were added the same volume of propylene glycol. The PMN in each group were respectively cultivated for 24h and 48h. Flow cytometry was adopted to detect the apoptosis rate of PMN. Results After being cultivated for 24h, when the concentration of Ginsenoside Rd was 100~mol/L, the apoptosis rate PMN was signifi- cantly lower than the control group （ P 〈 0.05 ）. After being cultivated for 48h, when the concentration of Ginsenoside Rd was 100μmol/ L, the apoptosis rate of PMN in the first group and the third group significantly reduced （P 〈 0.05 ）. There was no significant difference in the second group. Conclusion Ginsenoside Rd in high concentration can inhibit the apoptosis of normal and PMN in TB infection model, improve survival rate of cells and extend the survival time of cells.

关 键 词：人参皂甙RD 中性粒细胞 结核 凋亡 

分 类 号：R3[医药卫生—基础医学]