心复力颗粒对阿霉素致心力衰竭大鼠PPAR-α及ET-1的影响  

Effects of Xinfuli Granula on PPAR-α and ET-1 in Rats with Heart Failure Induced by Adriamycin

作　　者：郭彩霞[1] 马丽红[1] 沈启明[1] 张晓丽[1] 王少霞[2] 李扬[2] 

机构地区：[1]中国医学科学院/北京协和医学院国家心血管病中心,阜外心血管病医院,心血管疾病国家重点实验室,100037 [2]中国人民解放军军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《医学研究杂志》2015年第5期41-44,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81071177)

摘　　要：目的探讨心复力颗粒对阿霉素(adriamycin,ADR)致心力衰竭(heart failure,HF)大鼠PPAR-α及ET-1的影响。方法 60只雄性SD大鼠随机分为正常组,模型组,心复力颗粒低、中、高剂量组。除正常组外均给予ADR2.5mg/kg腹腔注射制备大鼠HF模型,正常组腹腔注射等量生理盐水,每周1次,持续6周。注射ADR第5周开始各组大鼠灌胃给药,将心复力颗粒用生理盐水配成0.5g/ml的混悬液,低、中、高剂量组的灌胃剂量分别为0.675g/(kg·d)、1.350g/(kg·d)、2.700g/(kg·d),正常组和模型组给予与中剂量组等体积生理盐水,每天1次,持续4周。用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)测定血浆中内皮素-1(endothelin-1,ET-1)含量,半定量RT-PCR法检测过氧化物酶体增生物激活受体(peroxisome proliferator activated receptor-α,PPAR-α)mRNA的表达。结果与正常组比较,模型组PPAR-αmRNA表达明显降低(P<0.05),ET-1蛋白表达明显升高(P<0.05);与模型组比较,心复力颗粒低、中、高剂量组PPAR-αmRNA显著升高(P<0.05);中、高剂量组ET-1蛋白表达显著降低(P<0.05),但心复力低剂量组与模型组ET-1蛋白表达无统计学差异(P>0.05)。结论心复力颗粒能明显改善HF大鼠心肌能量代谢,其机制可能与调节PPAR-α及ET-1的表达有关。Objective To investigate the effects of Xinfuli granula on PPAR - ctand ET - 1 in rats with heart failure（HF）. Meth- ods Sixty male SD rats were randomly divided into 5 groups： normal control group, HF group, low - dose xinfuli granula group, medium - dose xinfuli granula group and high - dose xinfuli granula group. All the groups were injected intraperitoneally with adriamyein except normal control group with isovolumic normal saline. All groups were given the corresponding drugs by intragastric injection every morning for 4weeks since the fifth week. Xinfuli granula was suspended by isovolumic normal saline with the concentration of 0.5g/ml, and the do- ses of xinfuli granula groups were 0. 675g/（ kg· d）, 1. 350g/（ kg · d） and 2. 700g/（ kg · d）, respectively. Normal control group and HF group were perfused with isovolumic normal saline as the same volume of medium - dose xinfuli granula group. Endothelin - 1 （ ET - 1 ） was measured by enzyme - linked immunosorbent assay （ELISA） , and the expressions of peroxisome proliferator activated receptor -α （ PPAR - α） was measured by RT - PCR. Results Compared with the normal control group, The expression of PPAR - ctdeereased sig- nificantly and the expression of ET - 1 increased in the HF group（ P 〈 O. 05 ）. Compared with the HF group, the PPAR - α expression was much higher in all three xinfuli granula groups （ P 〈 O. 05 ）. The ET - 1 expression was down - regulated obviously in medium - dose and high -dose xinfuli granula groups compared with the HF group（P 〈 0.05）. But the ET- 1 expression in low- dose xinfuli granula group had no significant diference relative to the HF group（P 〉 0.05）. Conclusion Our findings suggest that xinfuli granula can improve the cardiac function and myocardial energy metabolism in the HF rats induced by adriamycin, and the underlying mechanism may be related to its effects on the expression of PPAR - αand ET - 1.

关 键 词：过氧化物酶体增生物激活受体-α心力衰竭 心复力颗粒 能量代谢 

分 类 号：R2[医药卫生—中医学]