胆汁分离携带Panton valentin杀白细胞基因金黄色葡萄球菌流行情况及SCCmec分型  

Prevalence and SCCmec Typing of Panton Valentin Leukocidin Genes in Staphylococcus Aureus Isolated from Bile in the Gastroenterology Department

作　　者：应孙科 曹寒沁[2] 吴敏瑾[2] 金胜航[3] 

机构地区：[1]浙江省永康市第一人民医院检验科,321300 [2]浙江大学医学院附属第一医院检验科,杭州310003 [3]浙江大学医学院附属第二医院检验科,杭州310009

出　　处：《医学研究杂志》2015年第5期123-126,共4页Journal of Medical Research

摘　　要：目的调查消化内科患者胆汁分离携带Panton valentin杀白细胞基因的金黄色葡萄球菌流行情况及SCCmec分型。方法收集浙江大学医学院附属第一医院2008年7月～2013年7月消化内科患者815名分离的金黄色葡萄球菌75株，采用VITEK2COMPACT全自动微生物分析仪分析其药敏结果，采用PCR检测PVL基因，采用多重PCR对所有耐甲氧西林金黄色葡萄球菌的SCCmec分型。结果消化内科金黄色葡萄球菌的感染率为9．2％，25例为社区获得性感染，其PVL阳性菌株16例，占64．0％；50例为医院获得性感染，其中PVL阳性菌株11例，占22％。SCC分型显示以SCCmecnl型为主，占43．3％（13／30），SCCmecⅡ型2株，SCCmec1V型10株，未检出SCCmecI型和V型。16株PVL基因阳性MRSA菌株中SCCmecnl型有9株（56．25％），IV型有6株（37．5％），另外有1株未成功分型。结论消化内科胆汁分离的金黄色葡萄球菌PVL基因携带及分子流行情况显示主要以SCCmec11I型为主，携带PVL基因率较高，存在区域性流行的特点。Objective To investigate the prevalence and SCCmec typing of Panton valentin leukocidin genes in Staphylococcus au- reus isolated from bile in the gastroenterology department. Methods Seventy -five Staphylococcus aureus isolated from 815 patients in the gastroenterology department July 2008 to July 2013. We used the VITEK 2 COMPACT automatic microbial analyzer to determine the susceptibility to all the antimicrobial drugs. Additionally, we detected the PVL gene by PCR, and detected the SCCmec type of the MRSA strains by multiplex PCR. Results The infection rate of Staphylococcus aureus in gstroenterology department was 9.2%. There were 25 cases of community - acquired infections, which contained 16 cases PVL - positive strains, accounting for 64.0%. There were 50 cases of hospital - acquired infections, which contained 11 cases of PVL - positive strains, accounting for 22.0%. Among the MRSA isolates, 43.3% （13/30） were SCCmec Ill, 33.3% （10/30） were SCCmec IV, and 2 were SCCmee 11. Five strains were non- genotyping. None of the isolates were genotyped as SCCmec I or V . However, among the 16 PVL - positive MRSA strains, 56.25% （9/16） were SC- Cmec Ⅲ , 37.5% （6/16）were SCCmecIV. In addition, one strain was not successful genotyping. Conclusion The PVL gene detection and molecular epidemiology of Staphylococcus aureus isolated from bile in the gastroenterology department showed that the SCCmec Ⅲ was the main genotype and the rate of PVL genes was high, with a regional popular feature.

关 键 词：金黄色葡萄球菌 胆汁 杀白细胞基因 SCCmec分型 

分 类 号：R393[医药卫生—基础医学]