急性髓系白血病CDX2和β-catenin基因表达的相关性  

Expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia

作　　者：王巍[1,2] 孟灿[1,2] 刘佳[1,2] 杨志刚[1,2] 

机构地区：[1]广东医学院血液病研究室,广东湛江524000 [2]广东医学院附属医院血液内科,广东湛江524000

出　　处：《南方医科大学学报》2015年第5期728-732,共5页Journal of Southern Medical University

基　　金：广东省自然科学基金(S2012040006822);广东省医学科研基金(A2012419);东莞市高等院校;科研机构科技计划项目(2012108102041);广东医学院博士启动基金(XB1017);湛江市科技攻关项目(2010C3104010)

摘　　要：目的研究急性髓系白血病(AML)患者CDX2与β-catenin基因表达的相关性。方法实时定量PCR方法检测92例初诊AML、30例非恶性血液病患者(对照)CDX2及β-catenin m RNA表达,Western blot检测其中的26例AML、8例非恶性血液病患者CDX2、β-catenin蛋白表达。结果 AML组CDX2 m RNA和蛋白表达阳性率分别为84.78%和76.92%,对照组未检测到表达(P<0.01);β-catenin m RNA和蛋白在两组均表达,但AML组显著高于对照组(P<0.01)。CDX2、β-catenin m RNA表达均与初诊时白细胞数、LDH水平呈正相关(P<0.01)。AML患者获得完全缓解时,CDX2 m RNA转阴、β-catenin m RNA表达显著下降,复发时两者均增高。AML患者CDX2与β-catenin无论在m RNA和蛋白水平表达均呈显著正相关(P<0.01)。结论 AML患者存在CDX2基因过表达及Wnt/β-catenin信号通路激活,且CDX2与β-catenin表达呈显著正相关。Objective To investigate the expressions of CDX2 and β-catenin and their correlation in acute myeloid leukemia （AML）. Methods Real-time PCR was used to examine the expressions of CDX2 andβ-catenin mRNA in 92 de novo AML and 30 non-malignant patients （control）, and Western blot was employed to detect CDX2 andβ-catenin protein expressions in 26 de novo AML and 8 non-malignant patients. Results The positivity rates of CDX2 mRNA and protein expressions in AML group were 84.78% and 76.92%, respectively, but no CDX2 mRNA or protein expression were detected in control group （P〈0.01）. The expressions ofβ-catenin mRNA and protein were detected in all the patients in both groups but its expressions in AML group were significantly higher （P〈0.01）. The expressions of CDX2 and β-catenin mRNA were significantly correlated with WBC counts and LDH levels （P〈0.01）. The expression of CDX2 mRNA was not detected and the expression ofβ-catenin mRNA was decreased to normal level in AML patients with complete remission, while the expressions of CDX2 andβ-catenin mRNA were increased again in those with disease relapse. There were significantly positive correlations between CDX2 andβ-catenin expressions at both mRNA and protein levels （P〈0.01）. Conclusion Up-regulation of CDX2 gene and activation of Wnt/β-catenin pathway co-exist in AML patients and the expressions of CDX2 andβ-catenin are positively correlated.

关 键 词：急性髓系白血病 CDX2 Β-CATENIN 基因表达 

分 类 号：R733.71[医药卫生—肿瘤]