深低温停循环下幼猪脑皮质HIF-1与NGB的表达及作用  

Expression of hypoxia-inducible factor 1 and neuroglobin in piglet cortex during deep hypothermic circulatory arrest

作　　者：梁孟亚[1] 唐志贤[1] 陈光献[1] 荣健[1] 戴刚[1] 吴钟凯[1] 

机构地区：[1]中山大学附属第一医院心脏外科,广东广州510080

出　　处：《中国病理生理杂志》2015年第5期823-827,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81400307)

摘　　要：目的:观察深低温停循环(DHCA)下幼猪脑皮质缺氧诱导因子1(HIF-1)与神经珠蛋白(NGB)的表达变化。方法:五指山猪共15头随机分成体外循环组(CPB组)、深低温停循环组(DHCA组)与深低温停循环选择性顺行脑灌注组(SACP组)。建立体外循环后,DHCA组降温至18℃后停循环40 min,SACP组停循环后经无名动脉顺行脑灌注40 min。复灌180 min后取脑皮质组织行HE染色镜检,Western blot及real-time PCR检测HIF-1α、NGB蛋白表达及mRNA水平。结果:HE染色显示与DHCA组相比SACP组脑组织损伤显著减轻。复灌180min后DHCA组及SACP组脑皮质中HIF-1α的蛋白及mRNA水平均显著高于CPB组(P<0.05),同时SACP组动物脑组织HIF-1α的蛋白及mRNA水平显著高于DHCA组(P<0.05)。复灌180 min后DHCA组及SACP组脑皮质中NGB的蛋白及mRNA水平均显著高于CPB组(P<0.05),同时SACP组动物脑组织NGB的蛋白及mRNA水平显著高于DHCA组(P<0.05)。结论:HIF-1与NGB的表达上调参与了脑组织对DHCA脑损伤的反应机制,并可能是SACP脑保护作用的机制之一。AIM：To observe the expression of hypoxia-inducible factor 1 （HIF-1） and neuroglobin （NGB） in piglet cortex during deep hypothermic circulatory arrest.METHODS：Wuzhishan piglets were randomly assigned to car-diopulmonary bypass group （ CPB group） , 40 min of circulatory arrest （ CA） at 18 ℃ without cerebral perfusion （ DHCA group） or with selective antegrade cerebral perfusion （ SACP group） .After 180 min of reperfusion, cortical tissue was har-vested for determining HIF-1αand NGB expression by HE staining, Western blot and real-time PCR.RESULTS：Severer cerebral injury was observed in DHCA group than that in SACP group.After 180 min of reperfusion, HIF-1αprotein and mRNA levels were significantly higher in DHCA group than those in CPB group （P〈0.05）.Accordingly, SACP animal had higher levels of HIF-1αprotein and mRNA than those in DHCA group （P〈0.05）.Simultaneously, higher NGB pro-tein and mRNA levels were found in DHCA group than those in CPB group after 180 min of reperfusion （ P〈0.05） .The SACP animal had higher levels of NGB protein and mRNA than those in DHCA group （P〈0.05）.CONCLUSION：Up-regulation of HIF-1 and NGB are involved in the mechanism against cerebral injury resulting from DHCA in the cortex and possibly a part of cerebral protective effect of SACP.

关 键 词：深低温停循环 选择性顺行脑灌注 缺氧诱导因子1 神经珠蛋白 

分 类 号：R654.1[医药卫生—外科学] R363.2[医药卫生—临床医学]