温和灸对溃疡性结肠炎小鼠IL-2、TGF-β的影响  

Warming moxibustion increases serum levels of interleukin-2 and transforming growth factor beta in ulcerative colitis mice

作　　者：孙军刚[1,2] 耿煜[1] 吴巧凤[1] 王程玉林 杨惠[1] 张何骄子 王祥[1] 余曙光[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川省成都市610075 [2]四川省中西医结合医院预防保健科,四川省成都市610041

出　　处：《世界华人消化杂志》2015年第13期2058-2063,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81373737;教育部霍英东青年教师基础研究基金资助项目;No.141041;四川省科技厅青年基金资助项目;No.2015JQO058~~

摘　　要：目的:本研究利用动物实验探讨温和灸法对葡聚糖硫酸钠(dextran sulfate sodium salt,DSS)溃疡性结肠炎(ulcerative colitis,UC)模型小鼠肠道病理形态及血清中细胞因子白介素-2(interleukin-2,IL-2)、转化生长因子-β(transforming growth factorβ,TGF-β)影响,探讨温和灸治疗UC的相关免疫机制.方法:36只体质量在34 g±2 g的♂昆明小鼠(KM)随机分为空白组、模型组、模灸组.采用3%DSS连续7 d自由饮用法复制UC模型,用温和灸法从造模第5天开始选用关元、足三里(右侧),关元、足三里(左侧)交替连续治疗5 d,每次10 min.造模及治疗结束后比较小鼠疾病活动指数(disease activity index,DAI).治疗结束后剖取小鼠远端结肠组织,光学显微镜和透射电镜观察结肠组织病理形态变化,取动物血清,运用ELISA法检测血清中IL-2、TGF-β的含量.结果:造模结束后,模型组、模灸组DAI明显增高,两组之间差异无统计学意义(P>0.05).治疗结束后,模灸组DAI明显降低,与模型组比较差异有统计学意义(P<0.05).光镜下、电镜下空白组、模型组、模灸组形态结构有明显差异,模型组腺体结构破坏,黏膜及黏膜下层水肿、炎细胞浸润,微绒毛稀疏,杯状细胞明显增多,线粒体肿胀,细胞内存在大量空泡;模灸组组织细胞形态接近空白组.治疗结束后对比空白组,模型组血清中IL-2、TGF-β含量明显降低,模灸组稍增高,空白组及模型组比较差异有统计学意义(P<0.05),空白组与模灸组比较差异无统计学意义(P>0.05),模型组与模灸组比较差异有统计学意义(P<0.05).结论:温和灸可以明显改善UC模型小鼠受损肠黏膜的形态及升高血清中的IL-2、TGF-β含量,说明艾灸具有通过抗炎和调节免疫反应的作用发挥治疗UC的作用.AIM： To explore the effect of warming moxibustion on serum levels of interleukin-2 （IL-2） and transforming growth factor （TGF-β） in ulcerative colitis mice. METHODS： Thirty-six male Kunming mice were randomly divided into a control group, a model group and a warming moxibustion group. Ulcerative colitis was induced by treatment with 3% dextran sulfate sodium salt （DSS） for 7 d. The warming moxibustion group received treatment at ＂Zusanli＂ and ＂Guanyuan＂ acupoints for 5 d. Disease activity index （DAI） score was evaluated in the three groups. Tissue morphology and ultrastructure were evaluated by light microscopy and transmission electron microscopy （TEM）, respectively. Mouse blood samples were taken to test serum levels of IL-2 and TGF-β by enzyme-linked immunosorbent assay （ELISA）. RESULTS： Compared with the control group, DAI score was significantly increased, inflammatory cells were seen in the intestinal mucosal, and serum levels of IL-2 and TGF-β decreased significantly in the model group （P 〈 0.05）. Compared with the model group, DAI score was decreased significantly, intestinal mucosal structure and micro-structures were improved, and serum levels of IL-2 and TGF-β were significantly increased in the warming moxibustion group （P 〈 0.05）.CONCLUSION： Warming moxibustion inhibits the inflammation reactions in UC, promotes the repair of the intestinal mucosa, and increases the levels of serum [L-2 and TGF-β, which indicates that warming moxibustion exerts a therapeutic effect on UC by regulating IL-2 and TGF-β levels.

关 键 词：温和灸 溃疡性结肠炎 白介素-2 转化生长因子-Β 

分 类 号：R245[医药卫生—针灸推拿学]