不同雌孕激素水平对子宫内膜上皮细胞Claudin-4表达的意义  

Correlation of estrogen and progesterone levels with Claudin-4 expression in endometrial epithelial cells

作　　者：周围[1] 佘立平[1] 纪亚忠[1] 石礼红[1] 董胜男[2] Dhruba Paudel 

机构地区：[1]同济大学附属同济医院生殖医学科,上海200065 [2]同济大学附属同济医院妇产科,上海200065

出　　处：《同济大学学报（医学版）》2015年第2期45-48,52,共5页Journal of Tongji University(Medical Science)

基　　金：上海市卫生局项目(2011Y059)

摘　　要：目的观察不同雌、孕激素水平对子宫内膜上皮细胞Claudin-4表达的影响。方法 RL95-2细胞系培养于含10%胎牛血清培养基中,在培养基中加入雌、孕激素,一组孕酮的浓度恒定为0.025μmol/L,雌二醇终浓度分别为1、0.5、0.25、0.125、0.062 5和0 nmol/L;另一组雌二醇的浓度恒定为0.25 nmol/L,孕酮终浓度分别为0.1、0.05、0.025、0.012 5、0.006 25和0μmol/L。两组分别培养24、48和72 h,RT-PCR和Western印迹法检测RL95-2细胞的Claudin-4基因和蛋白表达。结果在雌激素恒定的条件下,RL95-2细胞的Claudin-4基因和蛋白表达随着孕激素水平的升高而升高(P<0.01),随着培养时间的延长,其表达Claudin-4基因和蛋白水平升高(P<0.01)。在孕激素恒定的条件下,RL95-2细胞的Claudin-4基因和蛋白表达随着雌激素水平的升高而降低(P<0.01),随着培养时间的延长,其表达Claudin-4基因和蛋白水平降低(P<0.01)。结论 Claudin-4在子宫内膜细胞的表达水平与雌孕激素的水平具有密切的联系,Claudin-4可能参与子宫容受性的形成和胚泡在子宫的着床有关。Objective To investigate the association of estrogen and progesterone levels with Claudin-4 expression in endometrial epithelial cells. Methods Endometrial epithelial RL95-2 cells were cultured in 10% FBS-containing medium, and different concentrations of estrogen and progesterone were added into the culture medium. When the concentration of progesterone maintained at 0. 025 μmol / L,the final concentrations of estradiol were 1,0. 5,0. 25,0. 125,0. 062 5 and0 nmol / L; or when the estradiol concentration maintained at 0. 25 nmol / L,the final concentration of progesterone were 0. 1,0. 05,0. 025,0. 012 5,0. 006 25 and 0 μmol / L,RL95-2 cells were cultured for 24 h,48 h and 72 h,respectively,the expression of Claudin-4 mRNA and protein was detected by RT-PCR and Western blotting. Results Under constant concentration of estrogen,the expression of Claudin-4 mRNA and protein in RL95-2 cells was increased as the progesterone levels increased or as the culture time was elongated（ P 〈0. 01）. Under constant conditions of progesterone,the expression of Claudin-4 mRNA and protein was decreased in RL95-2 cells as estrogen levels increased or as the culture time was elongated（ P 〈0. 01）. Conclusion The expression of Claudin-4 in endometrial epithelial cells is closely correlated with estrogen and progesterone levels,indicating that Claudin-4 may be involved in the formation of uterine receptivity and embryo implantation.

关 键 词：雌激素 孕激素 紧密连接 子宫内膜 

分 类 号：R711[医药卫生—妇产科学]