大黄酸对肿瘤坏死因子-α诱导的脐静脉内皮细胞黏附作用影响的观察  

Effect of Rhein on adhesive function of endothelial cells induced by TNF-α

作　　者：蒋荣[1] 张倩倩[1] 张久丹 卢斌[1] 臧璞[1] 刘隽[1] 柏晓勇[1] 杜宏[1] 

机构地区：[1]南京大学医学院临床学院内分泌科(南京军区总医院内分泌科),210002

出　　处：《中国糖尿病杂志》2015年第6期546-549,共4页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81173622);江苏省自然科学基金(BK2011664)

摘　　要：目的观察不同剂量的大黄酸(Rhein)对TNF-α诱导的人脐静脉内皮细胞(HUVECs)黏附作用的影响。方法将HUVECs分为单纯HUVECs组、单纯TNF-α40ng/ml(TNF-α)组、Rhein100μg/ml+TNF-α40ng/ml(Rhein100)组、Rhein 50μg/ml+TNF-α40ng/ml(Rhein50)组、Rhein 10μg/ml+TNF-α40ng/ml(Rhein10)组。采用Western blot和RT-PCR检测HUVECs细胞间黏附因子-1(ICAM-1)的蛋白和mRNA的水平。采用人单核细胞株(THP-1)和HUVECs的黏附性实验检测内皮细胞的黏附功能。结果与TNF-α组比较,Rhein100、Rhein50、Rhein10组ICAM-1蛋白相对表达量下降[(2.88±0.04)vs(1.27±0.32),(1.28±0.17),(1.32±0.25),P<0.05或P<0.01];Rhein100和Rhein50组ICAM-1mRNA的水平下降[(2.12±0.24)vs(1.19±0.17),(1.26±0.23),P<0.05或P<0.01];在黏附性实验中,Rhein100组HUVECs所黏附的单核细胞数目下降[(1.28±0.07)vs(1.07±0.14),P<0.01]。结论Rhein能抑制TNF-α所诱导的HUVECs的ICAM-1的过度表达,可能是Rhein对HUVECs的保护机制之一。Objective To examine the effects of various concentrations of Rhein on adhesive function of human umbilical vein endothelial cells（HUVECs）induced by tumor necrosis factor-α（TNF-α）.Methods HUVECs were divided into five groups：only HUVECs（HUVECs group）,TNF-α（treated with TNF-α40ng/ml）,Rhein100（treated with Rhein 100μg/ml plus TNF-α40ng/ml）,Rhein50（treated with Rhein 50μg/ml plus TNF-α40ng/ml）,Rhein10（treated with Rhein 10μg/ml plus TNF-α40ng/ml）.The mRNA and protein expressions of intercellular adhersionmolecule-1（ICAM-1）were detected by RTPCR and Western blot respectively,the adhesive function of HUVECs was detected by monocytic THP-1cells.Results Compared with TNF-αgroup,the expression of ICAM-1protein was decreased in Rhein100,Rhein 50,and Rhein 10group[（2.88±0.04）vs（1.27±0.32）,（1.28±0.17）,（1.32±0.25）,P〈0.05 or P〈0.01];the levels of ICAM-1mRNA were decreased in Rhein 100 and Rhein 50 group [（2.12±0.24）vs（1.19±0.17）,（1.26±0.23）,P〈0.05 or P〈0.01],and the number of adhesive monocytic THP-1cells was decreased in Rhein 100 group[（1.28±0.07）vs（1.07±0.14）,P 〈0.01].Conclusion Rhein can inhibit TNF-αinduced ICAM-1 expression in HUVECs,which is a potential mechanism for Rhein associated vascular protection.

关 键 词：大黄酸 人脐静脉内皮细胞 细胞间黏附因子-1 

分 类 号：R285[医药卫生—中药学]