1%特比萘芬乳膏对常见皮肤癣菌、球形孢子丝菌及裴氏着色真菌的体外抑菌活性研究  

Comparison of the antifungal activity of 1% terbinafine cream,2% ketoconazole cream and 1% bifonazole cream: an in vitro study

作　　者：尹斌[1,2] 冉玉平[1] 

机构地区：[1]四川大学华西医院皮肤性病科,四川成都610041 [2]成都市第二人民医院皮肤科,四川成都610017

出　　处：《皮肤病与性病》2015年第3期130-133,共4页Dermatology and Venereology

基　　金：北京诺华制药有限公司资助

摘　　要：目的评估外用抗真菌药膏对常见皮肤癣菌及球形孢子丝菌、裴氏着色真菌的体外抑菌活性。方法纳入皮肤常见致病真菌临床分离株7种19株,制作含菌琼脂平板,采用改良琼脂扩散法加入实验药膏,适当培养,观察菌株生长情况并测定抑菌圈直径。结果 1%特比萘芬乳膏对所有实验菌株均形成抑菌圈,对常见皮肤癣菌、球形孢子丝菌(酵母相与菌丝相)和裴氏着色真菌的抑菌圈直径大于2%酮康唑、1%联苯苄唑乳膏(P<0.05);1%联苯苄唑乳膏对于球形孢子丝菌、断发毛癣菌无抑菌圈。结论对于常见皮肤癣菌、球形孢子丝菌和裴氏着色真菌,1%特比萘芬乳膏较2%酮康唑乳膏、1%联苯苄唑乳膏的抗真菌谱更广,抗真菌活性更强。本研究所采用的改良琼脂扩散法成本低,重复性好,简单易操作、贴近临床实际,可为外用抗真菌药物的临床应用提供客观科学的实验依据。Objective To compare in vitro antifungal activities of three topical antifungal creams on common dermatophytes, Sporothrix globosa and Fonsecaea pedrosoi. Methods Nineteen pathogenic fungi from seven clinically isolated species were used in the study. A modified agar diffusion assay was performed by the modified disk diffusion susceptibility method. Growth status of the isolates and the diameters of the inhibition zones around drug-containing wells were recorded by using photography following an incubation period. Results The inhibition zones of 1% terbin- aline cream on common dermatophytes, Sporothrix globosa and Fonsecaea pedrosoi were significantly larger than those of the other two creams. No inhibition zones were observed around the 1% bifonazole cream in S globosa and Tricho- phyton tonsurans. Conclusion The 1% terbinafine cream was more effective in treating common dermatophytes than 1% bifonazole or 2% ketoconazole creams. The modified agar diffusion method is a simple, reproducible, cheap and easily adaptable assay for clinical use.

关 键 词：体外抗菌活性 改良琼脂扩散法 特比萘芬 外用抗真菌药物 

分 类 号：R978.5[医药卫生—药品] R969.4[医药卫生—药学]