HPLC-DAD同时测定黄芪生脉饮中4种成分含量  

Simultaneous Determination of Four Components in Huangqi Shengmai Decoction by HPLC-DAD

作　　者：陆铖[1,2] 周盛会 李炜[2] 郑修丽[2] 高庆剑 汤浩[2] 

机构地区：[1]宁夏医科大学药学院,宁夏银川750000 [2]甘肃省人民医院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2015年第7期96-99,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的：采用高效液相色谱法建立同时测定黄芪生脉饮中五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元含量的方法。方法色谱柱：Agilent Eclipse C18（4.6 mm＆#215;250 mm,5μm）；柱温：30℃；流动相：乙腈-0.1%冰醋酸水溶液,梯度洗脱；流速：1.0 mL/min；检测波长：280 nm。结果五味子乙素、五味子酯甲、党参炔苷和鲁斯可皂苷元的标准曲线线性关系良好,线性范围分别为0.8625～21.5625μg（r2＝0.9991）、0.7375～18.4375μg（r2＝0.9994）、0.0952～2.3800μg（r2＝0.9996）、0.8100～20.2500μg（r2＝0.9990）,平均回收率分别为98.06%、99.61%、97.98%、99.30%,RSD分别为1.64%、2.72%、1.45%、1.25%。结论该法准确可靠、快速、专属性强、结果稳定、重复性好。Objective To establish a method for simultaneous determination of contents of schisandrin B, schisantherin A, lobetyolin and ruscogenin in Huangqi Shengmai Decoction with HPLC method. Methods The chromatographic column was an Agilent Eclipse C18 column （4.6 mm × 250 mm, 5 μm） that was maintained at a constant temperature of 30 ℃. The mobile phase was 0.1% acetic acid solution-acetonitrile, with gradient elution. The flow rate was 1.0 mL/min. The detection was at wavelength of 280 nm. Results The standard curves of schisandrin B, schisantherin A, lobetyolin and ruscogenin had good linearity in the ranges of 0.862 5-21.562 5μg （r 2=0.999 1）, 0.737 5-18.437 5μg （r 2=0.999 4）, 0.095 2-2.380 0 μg （r 2=0.999 6）, and 0.810 0-20.250 0 μg （r 2=0.999 0）, respectively. The average recoveries of the four components were 98.06%（RSD=1.64%）, 99.61%（RSD=2.72%）, 97.98%（RSD=1.45%）, and 99.30%（RSD=1.25%）, respectively. Conclusion This method is accurate, rapid and specific, achieving the stable results with high reproducibility.

关 键 词：黄芪生脉饮 五味子乙素 五味子酯甲 党参炔苷 鲁斯可皂苷元 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]