AtHsfAla对拟南芥幼苗在干旱中生理指标的影响  

Effect of Heat Shock Factor AtHsfAla on Physiological Indexes in Arabidopsis Thaliana Seedlings in Drought

作　　者：谭越[1,2] 何承忠[2] 郭丽红[1] 

机构地区：[1]昆明学院云南省高校特色生物资源开发与利用重点实验室,云南昆明650214 [2]西南林业大学林学院,云南昆明650224

出　　处：《昆明学院学报》2015年第3期64-68,共5页Journal of Kunming University

基　　金：国家自然科学基金资助项目(31260061;31060039);校级课题资助项目(YJL11025);校级重点学科建设资助项目

摘　　要：以过量表达AtHsfAla及AtHsfAla基因沉默的转基因植株为材料,通过控制土壤水分进行干旱处理,结果显示,过量表达型干旱处理后的存活率最大,而基因沉默型最小,表明AtHsfA1a能增强植物的抗旱性.为了从生理水平研究AtHsfA1a提高抗旱性的机制,研究发现过量表达型的叶片相对含水量和叶绿素含量最高,而沉默型最低.不同基因型植株的H2O2和MDA的含量正好相反,说明AtHsfA1a通过保护细胞膜而减小细胞所受氧化伤害来提高抗旱能力.In this study,transgenic Arabidopsis thaliana plants with over expressing AtHsfA1a and silencing mutant of Arabidopsis AtHsfA1a were used as experimental material to be handled in drought by controlling the soil water.The results showed that the survival percents of the plants with over expressing AtHsfA1a were the highest,but the silencing mutant was the lowest,indicating that AtHsfA1a could enhance the drought resistance of Arabidopsis plants.In order to study the mechanism of drought resistance improved by heat shock factor AtHsfAla in physiological level,it was found that water content and chlorophyll content of the plants with over expressing AtHsfA1a were highest,while the silencing mutant were lowest.The contents of H2 O2 and MDA in different genotypes were just the opposite,showing that AtHsfA1a could reduce oxidative stress thus protect cell membranes from damage and enhance antioxidant capacity.

关 键 词：拟南芥 热激因子AtHsfAla 干旱胁迫 生理指标 

分 类 号：Q943.2[生物学—植物学]