红景天苷对大鼠局灶性脑缺血/再灌注损伤的神经保护作用  

Neuroprotective effects of salidroside against focal celebral ischemia/reperfusion injury in rats

作　　者：赖文芳[1,2] 张小琴[1] 洪海棉 谢秀利 洪桂祝 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122

出　　处：《中国药理学通报》2015年第6期775-780,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No 81473382);福建省科技厅重点项目(No 2014Y4004);福建省自然科学基金项目(No 2015J01328);福建中医药大学校管课题(No X2013010)

摘　　要：目的探讨红景天苷(salidroside,Sal)对大鼠局灶性脑缺血/再灌注损伤的神经保护作用及其机制。方法健康成年♂SD大鼠36只,随机分为3组:假手术组(sham)、模型对照组(MCAO组)、红景天苷给药组(MCAO+Sal组)。通过线栓法制作大鼠局灶性脑缺血/再灌注损伤模型,Longa评分法对大鼠神经功能损伤评分,焦油紫染色法对神经细胞内尼氏(Nissl)小体染色,RT-q PCR技术检测大鼠缺血侧脑组织Neun、Nogo-A与Ng R m RNA的表达,Western blot法检测大鼠缺血侧脑组织Bcl-2、Neun、BDNF、NGF、Nogo-A与Ng R蛋白的表达。结果与MCAO组比较,红景天苷能明显降低神经功能损伤,增加Nissl阳性细胞的数量,促进Bcl-2、Neun、NGF、BDNF的蛋白表达,降低Nogo-A及其受体Ng R的m RNA及蛋白表达。结论红景天苷能够降低MCAO模型大鼠神经功能损伤,增加Nissl阳性细胞数目,提高Neun的表达,具有神经保护作用,此作用是通过促进抗凋亡因子Bcl-2及神经营养因子NGF、BDNF的蛋白表达,下调轴突生长抑制因子Nogo-A及其受体Ng R的蛋白表达所实现。Aim To investigate the effect of Salidroside on the focal celebral ischemia/reperfusion injury in rats and its underlying mechanism. Methods Adult male Sprague-Dawley rats, weighing 260-300 g, were ran-domly divided into three groups： sham, MCAO, MCAO＋salidroside （ Sal ） groups. The rats were sub-jected to local celebral ischemia reperfusion with suture-occluded method. The rats of MCAO ＋Sal group were treated intraperitoneally with salidroside （ 50 mg ·kg^-1 ） for 6 days. Neurological deficit testing was performed with Longa’ s Scale. The mRNA expressions of Neun,Nogo-A,and NgR were detected by RT-qPCR in ischemic brain. The protein expressions of Neun, NGF , BDNF , Nogo-A and NgR were determined by Western blot. Results Compared with MCAO group, salidroside significantly improved the neurological defi-cit,promoted the expressions of Bcl-2,Neun,NGF,BD-NF, and inhibited the expressions of Nogo-A, NgR. Conclusion Salidroside can reduce neurological defi-cit, increase the number of Nissl’ s Body and the ex-pression of Neun, and protect rats against focal celebral ischemia/reperfusion injury,which may be accom-plished by increasing the expressions of Bcl-2, NGF, BDNF, and inhibiting the expressions of Nogo-A, NgR.

关 键 词：红景天苷 局灶性脑缺血/再灌注 轴突生长抑制因子 神经生长因子 神经保护 细胞凋亡 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]