CCK-8对足底电击诱导的吗啡CPP重燃的影响  

Effects of CCK-8 on foot shock primed reinstatement of morphine-induced CPP

作　　者：贾慧[1,2] 刘涛[3] 文迪[2] 孙东磊[2] 臧国庆[2] 于峰[2] 闫玉仙[1] 马春玲[2] 

机构地区：[1]武警后勤学院,天津300309 [2]河北医科大学基础医学院法医学系,河北省法医学重点实验室,河北石家庄050017 [3]云南省第二人民医院急诊内科,云南昆明650021

出　　处：《中国药理学通报》2015年第6期848-852,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81273337);河北省自然科学基金资助项目(No H2013206157)

摘　　要：目的建立吗啡条件性位置偏爱(CPP)模型,观察不同剂量的外源性CCK-8对足底电击诱导的吗啡CPP重燃的影响,并探讨其相关机制。方法建立足底电击诱导的吗啡CPP重燃模型,在CPP重燃前给予侧脑室注射CCK-8(0.1、1μg),以观察外源性CCK-8对足底电击诱导吗啡CPP重燃的影响。另外,CPP重燃前共同给予侧脑室注射CCK-8和CCK受体拮抗剂(CCK1受体拮抗剂L-364,718、CCK2受体拮抗剂L-365,260)、CCK-8和阿片受体拮抗剂(非选择性阿片受体拮抗剂纳洛酮、高选择性μ阿片受体拮抗剂CTAP),以探讨CCK-8抑制足底电击诱导吗啡CPP重燃的相关机制。结果 1足底电击成功重燃CPP的表达,并且0.1和1μg CCK-8有效抑制了CPP重燃过程。2 CCK1受体拮抗剂L-364,718和阿片受体拮抗剂纳洛酮、CTAP均可阻断CCK-8对足底电击诱导吗啡CPP重燃的抑制作用。结论 CCK-8可以通过CCK1受体抑制足底电击诱导的吗啡CPP重燃,并且这种作用与阿片受体相关。Aim To explore the effects of exogenous CCK-8 on foot shock primed reinstatement of morphine-induced CPP and its mechanism. Methods The mod-el of morphine induced conditioned place preference was established. Doses of CCK-8 （0. 1,1 μg） were given intracerebroventricularly before CPP reinstate-ment, and the effects of exogenous CCK-8 on foot shock primed reinstatement of morphine-induced CPP were examined. Furthermore, CCK receptor antago-nists （ CCK1 receptor antagonist L-364 ,718 and CCK2 receptor antagonist L-365 , 260 ） and opioid receptor antagonists （ opioid receptor antagonist naloxone andμ-opioid receptor antagonists CTAP） were co-administra-ted with CCK-8 before CPP reinstatement to explore the mechanisms of CCK-8 inhibiting foot shock primed re- instatement of morphine-induced CPP. Results ①Foot shock successfully provoked the reinstatement of CPP, and CCK-8 （0. 1,1 μg） effectively inhibited the reinstatement of morphine-induced CPP. ②CCK1 re-ceptor antagonist L-364 , 718 , opioid receptor antago-nists naloxone and CTAP could block the inhibitory effects of CCK-8 on foot shock primed reinstatement of morphine-induced CPP. ③ CCK-8 itself had no effect on sensitivity threshold of rats to foot shock. Conclu-sion CCK-8 can inhibit foot shock primed reinstate-ment of morphine-induced CPP via CCK1 receptor. This function of CCK-8 is related to opioid receptor.

关 键 词：吗啡 八肽胆囊收缩素 胆囊收缩素受体 复吸 条件性位置偏爱 内源性阿片肽 

分 类 号：R341.6[医药卫生—基础医学] R749.61